Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 124.94 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 815.68
Colo858-P9
D10
COLO 858 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 75.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 123.44 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 740.29
Colo858-P9
D11
COLO 858 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 654.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.86 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 681.06
Colo858-P9
D2
COLO 858 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 558.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 218.39 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 405.02
Colo858-P9
D3
COLO 858 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 439.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 208.10 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 562.52
Colo858-P9
D4
COLO 858 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 251.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.68 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 483.43
Colo858-P9
D5
COLO 858 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 293.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 180.96 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 792.68
Colo858-P9
D6
COLO 858 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 360.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 813.43
Colo858-P9
D7
COLO 858 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 247.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.05 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 359.05
Colo858-P9
D8
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 319.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.76 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 297.48
Colo858-P9
D9
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 147.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 61.29 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 375.03
WM115-P9
D10
WM-115 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 77.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 60.54 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 326.39
WM115-P9
D11
WM-115 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 386.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 79.15 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 308.21
WM115-P9
D2
WM-115 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 85.72 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 310.39
WM115-P9
D3
WM-115 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 379.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 88.99 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 354.48
WM115-P9
D4
WM-115 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 287.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 90.48 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 273.12
WM115-P9
D5
WM-115 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 496.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.83 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 616.06
WM115-P9
D6
WM-115 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 593.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 89.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 528.75
WM115-P9
D7
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 207.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 77.05 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 513.72
WM115-P9
D8
WM-115 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 268.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 67.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 359.09
WM115-P9
D9
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 385.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 151.89 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 363.22
A375-P3
G10
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 362.39 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 142.43 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 326.27
A375-P3
G11
A-375 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 494.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 129.01 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 542.75
A375-P3
G2
A-375 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 475.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 144.00 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 530.78
A375-P3
G3
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 367.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 145.41 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 738.84
A375-P3
G4