Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 599.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 201.31 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 130.11
Colo858-P4
F10
COLO 858 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 572.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.44 NGFR rabbit IgG(H+L) / donkey NGFR 1097.04
Colo858-P1
C5
COLO 858 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.71 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 294.18
Colo858-P4
C8
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 684.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.91 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 357.40
A375-P4
C2
COLO 858 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 284.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.94 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 204.79
Colo858-P4
C7
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 912.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 210.80 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 377.59
Colo858-P4
F3
COLO 858 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 326.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.90 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 250.06
Colo858-P4
C9
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 688.95 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.69 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 363.53
A375-P4
C3
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 232.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 123.94 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 304.95
A375-P4
C4
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 445.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.24 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 403.95
Colo858-P4
C4
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 894.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 192.23 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 331.75
Colo858-P4
C3
COLO 858 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 360.75 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.77 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 402.98
Colo858-P4
C5
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 971.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 199.36 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 94.58
Colo858-P4
C11
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 555.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.48 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 110.85
Colo858-P4
C10
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1193.30 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 206.40 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 350.18
Colo858-P4
C2
COLO 858 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 543.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.84 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 248.68
Colo858-P4
C6
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 411.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.50 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 209.21
A375-P9
F9
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 384.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 127.81 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 332.07
A375-P9
F8
A-375 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 940.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 181.09 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 405.15
A375-P9
F7
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 797.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 184.78 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 473.97
A375-P9
F6
A-375 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 602.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 170.12 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 361.62
A375-P9
F5
A-375 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 760.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 203.41 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 473.87
A375-P9
F4
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 540.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.39 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 337.65
A375-P9
F3
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 786.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 210.29 NGFR rabbit IgG(H+L) / donkey NGFR 822.61
A375-P1
C3
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 464.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 135.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 330.83
A375-P9
F2