Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 428.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 164.18 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1980.83
A375-P2
B5
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2084.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.10 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 190.22
A375-P10
B2
A-375 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2453.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 457.23 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7382.49
A375-P2
B6
COLO 858 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2264.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 242.71 NGFR rabbit IgG(H+L) / donkey NGFR 101.71
Colo858-P7
B6
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1907.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.84 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7872.32
A375-P2
B7
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2305.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 453.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 213.68
A375-P10
B3
A-375 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2470.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 465.05 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7713.06
A375-P2
B8
A-375 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1947.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 351.59 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 184.06
A375-P4
E6
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1538.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 307.16 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7914.56
A375-P2
B9
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1734.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 353.69 NGFR rabbit IgG(H+L) / donkey NGFR 119.95
A375-P1
E11
A-375 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1919.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 391.41 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 234.89
A375-P10
B4
A-375 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 798.61 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.50 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 224.54
A375-P9
B10
A-375 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1326.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 315.74 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 174.73
A375-P10
B5
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 823.97 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 170.97 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 134.09
A375-P4
E9
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3098.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 540.77 NGFR rabbit IgG(H+L) / donkey NGFR 567.23
A375-P1
E2
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1313.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 271.50 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 180.78
A375-P4
E7
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1461.59 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 333.92 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 205.97
A375-P10
B6
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2008.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 365.17 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 143.22
A375-P10
B8
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1278.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 266.10 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 287.39
A375-P9
B7
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2025.20 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 389.96 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 163.83
A375-P4
E10
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1529.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 298.67 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 142.92
A375-P4
E11
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2523.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 389.84 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 263.75
A375-P4
E2
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2565.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 412.30 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 260.81
A375-P4
E3
COLO 858 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2696.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 307.85 NGFR rabbit IgG(H+L) / donkey NGFR 109.04
Colo858-P7
E4
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3396.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 398.45 NGFR rabbit IgG(H+L) / donkey NGFR 132.06
Colo858-P7
E3