Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2353.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 495.34 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7462.55
A375-P8
B2
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3009.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 525.64 NGFR rabbit IgG(H+L) / donkey NGFR 196.08
A375-P7
E3
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1689.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 371.16 NGFR rabbit IgG(H+L) / donkey NGFR 370.45
A375-P7
E6
A-375 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1566.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 332.72 NGFR rabbit IgG(H+L) / donkey NGFR 283.67
A375-P7
E5
A-375 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2339.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 429.64 NGFR rabbit IgG(H+L) / donkey NGFR 360.80
A375-P7
E4
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2087.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 373.66 NGFR rabbit IgG(H+L) / donkey NGFR 130.03
A375-P7
E9
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2413.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 408.16 NGFR rabbit IgG(H+L) / donkey NGFR 329.47
A375-P7
E8
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3971.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 473.49 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 136.32
Colo858-P3
E10
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3639.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 456.21 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 112.58
Colo858-P3
E11
A-375 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 990.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 214.46 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 6076.04
A375-P8
B10
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 436.76 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.84 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 955.43
Colo858-P3
E4
COLO 858 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2380.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 306.62 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 186.68
Colo858-P3
E6
COLO 858 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1765.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 268.42 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 283.16
Colo858-P3
E7
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2384.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 478.03 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7901.41
A375-P8
B3
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2181.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 406.40 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 169.81
A375-P4
B10
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1686.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 314.79 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 140.38
A375-P4
B11
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2597.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 404.96 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 265.99
A375-P4
B2
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2409.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 391.32 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 254.09
A375-P4
B3
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 230.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.27 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 293.14
A375-P4
B4
A-375 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 260.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 134.67 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 350.66
A375-P4
B5
A-375 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2059.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 371.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 198.06
A375-P4
B6
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1527.20 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 297.11 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 183.05
A375-P4
B7
A-375 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1984.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 378.90 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 182.56
A375-P4
B8
COLO 858 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2017.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 307.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 141.78
Colo858-P4
B9
COLO 858 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2439.30 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 279.87 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 113.23
Colo858-P4
B8