Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1103.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 207.56 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 188.71
A375-P3
B9
COLO 858 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1683.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 315.88 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 157.68
Colo858-P4
E9
A-375 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2118.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 373.10 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 311.40
A375-P3
B8
WM-115 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 644.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 162.03 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 254.66
WM115-P3
E8
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 96.00 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 390.35
WM115-P3
D8
WM-115 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 258.39 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 93.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 177.11
WM115-P3
D9
WM-115 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.64 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 120.95
WM115-P3
E9
COLO 858 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 372.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 157.99 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 794.41
Colo858-P2
C8
A-375 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 424.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 142.27 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 309.39
A375-P2
G9
WM-115 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 416.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 128.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 457.06
WM115-P3
C8
A-375 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 448.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.13 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 635.30
A375-P2
G8
COLO 858 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.91 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 642.08
Colo858-P3
F8
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1339.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 253.18 NGFR rabbit IgG(H+L) / donkey NGFR 154.21
A375-P1
B9
WM-115 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 248.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 107.79 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 179.07
WM115-P3
C9
WM-115 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 385.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 144.82 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2995.29
WM115-P2
G9
COLO 858 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 465.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.37 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 505.86
Colo858-P3
F9
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 515.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.53 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 579.91
A375-P2
F9
COLO 858 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2223.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 320.01 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5074.68
Colo858-P2
B8
A-375 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 491.30 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.20 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1191.87
A375-P2
F8
COLO 858 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2416.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 295.01 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 178.42
Colo858-P3
E8
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 516.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.19 NGFR rabbit IgG(H+L) / donkey NGFR 493.56
A375-P1
C9
WM-115 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 321.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.06 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 3169.91
WM115-P2
F9
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 378.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 97.15 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1106.45
WM115-P2
G8
WM-115 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 681.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 225.77
WM115-P3
B8
COLO 858 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 460.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.93 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 514.91
Colo858-P3
D9