Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

 Full screen view
Filter results: >>>>
Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3260.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 400.81 NGFR rabbit IgG(H+L) / donkey NGFR 142.45
Colo858-P7
B2
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3073.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 581.89 NGFR rabbit IgG(H+L) / donkey NGFR 205.60
A375-P7
B3
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3359.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 399.82 NGFR rabbit IgG(H+L) / donkey NGFR 133.84
Colo858-P7
B3
WM-115 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 588.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.22 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 96.17
WM115-P10
B3
WM-115 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 478.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 113.15 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 193.46
WM115-P10
C2
WM-115 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 451.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.09 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 172.48
WM115-P10
D2
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2305.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 453.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 213.68
A375-P10
B3
COLO 858 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 499.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 170.39 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 249.37
Colo858-P8
G2
COLO 858 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1398.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 204.82 NGFR rabbit IgG(H+L) / donkey NGFR 191.82
Colo858-P7
D3
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2609.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 401.06 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 4949.40
Colo858-P8
B3
WM-115 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 960.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 204.29 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 125.96
WM115-P10
B2
WM-115 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 436.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 107.52 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 156.01
WM115-P10
D3
WM-115 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 875.59 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 196.54 NGFR rabbit IgG(H+L) / donkey NGFR 783.21
WM115-P7
E3
A-375 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1855.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 354.28 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 322.58
A375-P9
B4
A-375 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1276.95 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 295.45 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 259.89
A375-P9
B5
COLO 858 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 469.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 185.49 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 541.20
Colo858-P9
G4
COLO 858 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 214.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.19 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 273.03
Colo858-P10
F5
A-375 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 866.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 186.26 NGFR rabbit IgG(H+L) / donkey NGFR 661.46
A375-P7
F4
COLO 858 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 367.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 178.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 482.06
Colo858-P9
G5
COLO 858 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2047.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 286.72 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 162.29
Colo858-P9
E5
COLO 858 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 587.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 222.89 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 543.83
Colo858-P9
F4
A-375 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 882.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 203.27 NGFR rabbit IgG(H+L) / donkey NGFR 381.84
A375-P7
F5
A-375 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 575.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 188.67 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 212.07
A375-P10
G5
COLO 858 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2097.61 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 302.09 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 153.69
Colo858-P9
E4
COLO 858 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 373.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 157.06 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 491.11
Colo858-P9
F5