Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 614.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.13 NGFR rabbit IgG(H+L) / donkey NGFR 225.89
A375-P7
D3
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2419.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 504.37 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 8018.49
A375-P8
E3
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2084.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.10 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 190.22
A375-P10
B2
COLO 858 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 622.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 222.22 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 521.65
Colo858-P9
F3
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 540.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.39 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 337.65
A375-P9
F3
COLO 858 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 509.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.66 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 607.02
Colo858-P9
G2
COLO 858 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 539.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 192.62 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 415.11
Colo858-P9
G3
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 505.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 123.91 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 686.29
A375-P8
F2
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3009.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 525.64 NGFR rabbit IgG(H+L) / donkey NGFR 196.08
A375-P7
E3
A-375 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 426.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.28 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 299.21
A375-P9
G2
A-375 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 471.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 121.48 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 338.36
A375-P8
G2
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 583.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.10 NGFR rabbit IgG(H+L) / donkey NGFR 286.84
A375-P7
F2
COLO 858 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2484.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 351.08 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 109.58
Colo858-P9
E2
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2762.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 383.98 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 125.22
Colo858-P9
E3
A-375 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 536.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.84 NGFR rabbit IgG(H+L) / donkey NGFR 269.68
A375-P7
G2
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2305.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 453.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 213.68
A375-P10
B3
COLO 858 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1752.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 273.50 NGFR rabbit IgG(H+L) / donkey NGFR 335.70
Colo858-P7
F2
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 581.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 135.66 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 978.37
A375-P8
F3
COLO 858 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2300.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 334.83 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 116.61
Colo858-P10
E2
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 707.83 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 161.86 NGFR rabbit IgG(H+L) / donkey NGFR 226.02
A375-P7
F3
COLO 858 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 558.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 218.39 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 405.02
Colo858-P9
D3
COLO 858 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 664.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 220.19 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 549.42
Colo858-P9
F2
WM-115 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 478.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 113.15 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 193.46
WM115-P10
C2
WM-115 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 365.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 73.56 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 434.05
WM115-P8
G2
WM-115 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 468.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 102.70 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 843.37
WM115-P8
F3