Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2484.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 351.08 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 109.58
Colo858-P9
E2
A-375 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 507.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 154.56 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 478.41
A375-P8
D3
COLO 858 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2512.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 349.85 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 142.66
Colo858-P9
B2
A-375 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 536.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 145.29 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 352.65
A375-P8
D2
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2708.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 393.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 143.44
Colo858-P9
B3
COLO 858 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 499.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 170.39 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 249.37
Colo858-P8
G2
COLO 858 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 634.88 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 176.32 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 197.04
Colo858-P8
G3
COLO 858 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 828.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 231.09 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 648.00
Colo858-P9
C2
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 465.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.19 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 247.07
A375-P10
C3
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 553.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.05 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 233.07
A375-P10
C2
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 476.93 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.20 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 381.97
A375-P9
C3
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 536.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.82 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 375.04
A375-P9
C2
COLO 858 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 512.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 182.14 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 332.96
Colo858-P8
F3
COLO 858 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 544.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 202.11 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 427.01
Colo858-P9
C3
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 610.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.72 NGFR rabbit IgG(H+L) / donkey NGFR 258.83
A375-P7
C2
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 630.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.48 NGFR rabbit IgG(H+L) / donkey NGFR 213.50
A375-P7
C3
COLO 858 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2456.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 384.44 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 4756.87
Colo858-P8
E2
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 546.78 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.03 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 803.15
A375-P8
C3
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 593.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.33 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1088.62
A375-P8
C2
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2604.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 409.44 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5110.94
Colo858-P8
E3
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2781.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 536.04 NGFR rabbit IgG(H+L) / donkey NGFR 281.52
A375-P7
B2
COLO 858 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 652.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 197.39 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 185.41
Colo858-P8
D2
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2305.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 453.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 213.68
A375-P10
B3
A-375 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 494.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.18 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 368.49
A375-P9
D2
COLO 858 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 655.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 195.95 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 316.78
Colo858-P8
D3