Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 418.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.24 NGFR rabbit IgG(H+L) / donkey NGFR 1197.37
WM115-P1
G11
WM-115 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 555.30 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.25 NGFR rabbit IgG(H+L) / donkey NGFR 2417.00
WM115-P1
G2
WM-115 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 495.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 116.67 NGFR rabbit IgG(H+L) / donkey NGFR 2045.07
WM115-P1
G6
WM-115 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 473.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.67 NGFR rabbit IgG(H+L) / donkey NGFR 1519.03
WM115-P1
G7
WM-115 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 370.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 125.66 NGFR rabbit IgG(H+L) / donkey NGFR 1337.75
WM115-P1
G9
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 195.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 77.41 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 65.04
WM115-P10
E10
WM-115 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 865.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 188.48 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 121.83
WM115-P10
E2
WM-115 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 783.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 183.99 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 112.07
WM115-P10
E3
WM-115 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 535.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.41 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 138.23
WM115-P10
E5
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3740.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 484.10 NGFR rabbit IgG(H+L) / donkey NGFR 116.54
Colo858-P1
E10
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3548.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 455.96 NGFR rabbit IgG(H+L) / donkey NGFR 92.98
Colo858-P1
E11
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2368.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 298.19 NGFR rabbit IgG(H+L) / donkey NGFR 104.30
Colo858-P1
E2
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2555.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 349.95 NGFR rabbit IgG(H+L) / donkey NGFR 143.72
Colo858-P1
E3
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 613.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.35 NGFR rabbit IgG(H+L) / donkey NGFR 1111.88
Colo858-P1
E4
COLO 858 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 562.03 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.50 NGFR rabbit IgG(H+L) / donkey NGFR 1064.46
Colo858-P1
E5
COLO 858 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2275.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 321.46 NGFR rabbit IgG(H+L) / donkey NGFR 113.83
Colo858-P1
E6
COLO 858 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1665.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 240.80 NGFR rabbit IgG(H+L) / donkey NGFR 99.42
Colo858-P1
E7
COLO 858 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2460.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 338.45 NGFR rabbit IgG(H+L) / donkey NGFR 116.60
Colo858-P1
E8
COLO 858 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1882.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 354.07 NGFR rabbit IgG(H+L) / donkey NGFR 82.07
Colo858-P1
E9
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 718.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 187.45 NGFR rabbit IgG(H+L) / donkey NGFR 61.02
Colo858-P1
F10
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 922.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 217.82 NGFR rabbit IgG(H+L) / donkey NGFR 56.24
Colo858-P1
F11
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1188.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 232.45 NGFR rabbit IgG(H+L) / donkey NGFR 555.83
Colo858-P1
F2
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1228.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 356.29 NGFR rabbit IgG(H+L) / donkey NGFR 611.83
Colo858-P1
F3
WM-115 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 427.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 84.42 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 166.64
WM115-P10
F4
WM-115 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 495.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.79 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 166.97
WM115-P10
F3