Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 631.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 190.51 NGFR rabbit IgG(H+L) / donkey NGFR 45.27
Colo858-P1
D11
COLO 858 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 957.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 217.74 NGFR rabbit IgG(H+L) / donkey NGFR 769.46
Colo858-P1
D2
COLO 858 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1028.59 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 154.55 NGFR rabbit IgG(H+L) / donkey NGFR 643.21
Colo858-P7
F5
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 582.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.15 NGFR rabbit IgG(H+L) / donkey NGFR 1119.00
Colo858-P1
D4
COLO 858 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 509.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.92 NGFR rabbit IgG(H+L) / donkey NGFR 1008.58
Colo858-P1
D5
COLO 858 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 338.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.71 NGFR rabbit IgG(H+L) / donkey NGFR 272.57
Colo858-P1
D6
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1065.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 221.73 NGFR rabbit IgG(H+L) / donkey NGFR 534.00
A375-P7
F6
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 401.40 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.60 NGFR rabbit IgG(H+L) / donkey NGFR 422.47
Colo858-P1
D8
COLO 858 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 491.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 154.52 NGFR rabbit IgG(H+L) / donkey NGFR 126.90
Colo858-P1
D9
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3740.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 484.10 NGFR rabbit IgG(H+L) / donkey NGFR 116.54
Colo858-P1
E10
A-375 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 882.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 203.27 NGFR rabbit IgG(H+L) / donkey NGFR 381.84
A375-P7
F5
A-375 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 866.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 186.26 NGFR rabbit IgG(H+L) / donkey NGFR 661.46
A375-P7
F4
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 707.83 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 161.86 NGFR rabbit IgG(H+L) / donkey NGFR 226.02
A375-P7
F3
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 941.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 206.09 NGFR rabbit IgG(H+L) / donkey NGFR 778.64
A375-P1
F2
COLO 858 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 941.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 128.79 NGFR rabbit IgG(H+L) / donkey NGFR 401.37
Colo858-P7
F6
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 583.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.10 NGFR rabbit IgG(H+L) / donkey NGFR 286.84
A375-P7
F2
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 112.32 NGFR rabbit IgG(H+L) / donkey NGFR 272.43
A375-P7
F11
A-375 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 354.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 209.98 NGFR rabbit IgG(H+L) / donkey NGFR 635.87
A375-P7
F10
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2087.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 373.66 NGFR rabbit IgG(H+L) / donkey NGFR 130.03
A375-P7
E9
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 536.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.46 NGFR rabbit IgG(H+L) / donkey NGFR 300.18
A375-P1
D10
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2413.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 408.16 NGFR rabbit IgG(H+L) / donkey NGFR 329.47
A375-P7
E8
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1607.03 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 317.34 NGFR rabbit IgG(H+L) / donkey NGFR 319.81
A375-P7
E7
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1689.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 371.16 NGFR rabbit IgG(H+L) / donkey NGFR 370.45
A375-P7
E6
A-375 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1566.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 332.72 NGFR rabbit IgG(H+L) / donkey NGFR 283.67
A375-P7
E5
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 523.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 171.20 NGFR rabbit IgG(H+L) / donkey NGFR 138.23
A375-P1
F11