Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 517.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.94 NGFR rabbit IgG(H+L) / donkey NGFR 1172.91
A375-P1
F4
A-375 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 493.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.91 NGFR rabbit IgG(H+L) / donkey NGFR 1049.55
A375-P1
F5
A-375 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 544.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.98 NGFR rabbit IgG(H+L) / donkey NGFR 308.08
A375-P1
F6
A-375 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 492.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.59 NGFR rabbit IgG(H+L) / donkey NGFR 204.04
A375-P1
F7
A-375 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 497.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.72 NGFR rabbit IgG(H+L) / donkey NGFR 524.06
A375-P1
F8
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 475.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.33 NGFR rabbit IgG(H+L) / donkey NGFR 510.86
A375-P1
F9
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 468.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.69 NGFR rabbit IgG(H+L) / donkey NGFR 243.06
A375-P1
G10
A-375 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 506.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.46 NGFR rabbit IgG(H+L) / donkey NGFR 429.87
A375-P1
G9
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 471.76 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.07 NGFR rabbit IgG(H+L) / donkey NGFR 153.81
A375-P1
G11
A-375 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 667.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 168.31 NGFR rabbit IgG(H+L) / donkey NGFR 820.85
A375-P1
G2
A-375 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 692.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.65 NGFR rabbit IgG(H+L) / donkey NGFR 816.14
A375-P1
G3
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 519.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 151.01 NGFR rabbit IgG(H+L) / donkey NGFR 890.02
A375-P1
G4
A-375 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 555.54 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.74 NGFR rabbit IgG(H+L) / donkey NGFR 1086.87
A375-P1
G5
A-375 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 576.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 161.13 NGFR rabbit IgG(H+L) / donkey NGFR 458.75
A375-P1
G6
A-375 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 576.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.69 NGFR rabbit IgG(H+L) / donkey NGFR 331.16
A375-P1
G7