Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 882.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.39 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 210.67
WM115-P9
E7
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 114.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 69.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 383.22
WM115-P9
F10
WM-115 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 70.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 58.63 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 335.52
WM115-P9
F11
WM-115 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 479.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.79 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 317.57
WM115-P9
F3
WM-115 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 433.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 85.48 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 323.23
WM115-P9
F4
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 110.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 83.69 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 101.74
WM115-P9
B10
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 56.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 66.25 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 93.13
WM115-P9
B11
WM-115 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 647.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 140.20
WM115-P9
B3
WM-115 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 592.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.51 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 193.75
WM115-P9
B4
WM-115 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 847.88 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.75 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 204.30
WM115-P9
B7
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 821.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 214.74 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 180.19
WM115-P9
B8
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 69.37 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 398.64
WM115-P9
C10
WM-115 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 53.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 55.02 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 347.78
WM115-P9
C11
WM-115 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 404.40 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 285.66
WM115-P9
C5
WM-115 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 610.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 88.70 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 471.70
WM115-P9
C6
WM-115 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 250.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 82.81 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 485.42
WM115-P9
C8
WM-115 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 83.29 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 376.53
WM115-P9
C9
WM-115 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 386.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 79.15 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 308.21
WM115-P9
D2
WM-115 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 85.72 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 310.39
WM115-P9
D3
WM-115 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 287.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 90.48 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 273.12
WM115-P9
D5
WM-115 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 496.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.83 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 616.06
WM115-P9
D6
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 73.03 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 100.03
WM115-P9
E11
WM-115 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 712.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.78 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 136.83
WM115-P9
E2
WM-115 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 82.91 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 396.60
WM115-P9
F9
WM-115 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 308.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 84.15 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 475.73
WM115-P9
F8