Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

 Full screen view
Filter results: >>>>
Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1053.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 228.32 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 3110.03
WM115-P2
E2
COLO 858 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1450.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.96 NGFR rabbit IgG(H+L) / donkey NGFR 177.02
Colo858-P7
C8
COLO 858 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 654.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.86 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 681.06
Colo858-P9
D2
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1015.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.72 NGFR rabbit IgG(H+L) / donkey NGFR 506.01
A375-P7
C6
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 499.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 121.00
Colo858-P3
G11
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 892.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.01 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 314.05
A375-P10
C6
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 985.54 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 226.86 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 90.96
A375-P8
C6
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1053.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 226.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 692.16
Colo858-P3
F2
COLO 858 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1526.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 224.83 NGFR rabbit IgG(H+L) / donkey NGFR 82.51
Colo858-P7
E11
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 786.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 223.56 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 3331.33
WM115-P8
E8
COLO 858 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 587.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 222.89 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 543.83
Colo858-P9
F4
COLO 858 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 620.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 222.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 484.31
Colo858-P9
C4
COLO 858 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1375.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 222.63 NGFR rabbit IgG(H+L) / donkey NGFR 642.79
Colo858-P7
C4
COLO 858 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 622.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 222.22 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 521.65
Colo858-P9
F3
COLO 858 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1009.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 222.20 NGFR rabbit IgG(H+L) / donkey NGFR 765.25
Colo858-P7
D5
A-375 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 979.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 221.80 NGFR rabbit IgG(H+L) / donkey NGFR 400.84
A375-P7
C7
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1065.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 221.73 NGFR rabbit IgG(H+L) / donkey NGFR 534.00
A375-P7
F6
WM-115 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 693.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 220.97 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2715.98
WM115-P8
B9
COLO 858 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1344.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 220.89 NGFR rabbit IgG(H+L) / donkey NGFR 147.29
Colo858-P7
C3
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1147.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 220.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 651.77
Colo858-P3
C2
COLO 858 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 664.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 220.19 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 549.42
Colo858-P9
F2
COLO 858 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 800.78 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 220.10 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 4527.17
Colo858-P8
E11
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1020.88 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 220.00 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 297.91
WM115-P3
E3
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 986.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 219.91 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 678.93
Colo858-P3
F3
COLO 858 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 627.80 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 219.89 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 131.63
Colo858-P8
C4