Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3094.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 552.89 NGFR rabbit IgG(H+L) / donkey NGFR 593.97
A375-P1
B2
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3073.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 581.89 NGFR rabbit IgG(H+L) / donkey NGFR 205.60
A375-P7
B3
COLO 858 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3068.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 353.57 NGFR rabbit IgG(H+L) / donkey NGFR 101.45
Colo858-P7
B5
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3036.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 535.40 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 6341.83
A375-P2
B2
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3009.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 525.64 NGFR rabbit IgG(H+L) / donkey NGFR 196.08
A375-P7
E3
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2998.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 515.01 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5982.45
A375-P2
E3
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2988.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 526.60 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 6337.37
A375-P2
E2
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2981.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 513.45 NGFR rabbit IgG(H+L) / donkey NGFR 573.44
A375-P1
B3
COLO 858 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2934.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 367.66 NGFR rabbit IgG(H+L) / donkey NGFR 174.84
Colo858-P7
E8
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2927.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 487.18 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5940.11
A375-P2
B3
COLO 858 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2886.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 315.93 NGFR rabbit IgG(H+L) / donkey NGFR 78.49
Colo858-P7
E5
COLO 858 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2805.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 343.41 NGFR rabbit IgG(H+L) / donkey NGFR 118.72
Colo858-P7
B4
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2781.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 536.04 NGFR rabbit IgG(H+L) / donkey NGFR 281.52
A375-P7
B2
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2776.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 389.01 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 288.12
Colo858-P9
B9
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2762.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 383.98 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 125.22
Colo858-P9
E3
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2735.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 434.01
A375-P3
E2
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2728.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 495.24 NGFR rabbit IgG(H+L) / donkey NGFR 132.92
A375-P1
E10
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2722.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 510.86 NGFR rabbit IgG(H+L) / donkey NGFR 141.68
A375-P1
B10
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2708.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 393.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 143.44
Colo858-P9
B3
COLO 858 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2696.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 307.85 NGFR rabbit IgG(H+L) / donkey NGFR 109.04
Colo858-P7
E4
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2682.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 414.64 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 436.39
A375-P3
B2
COLO 858 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2658.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 378.09 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5377.62
Colo858-P2
B6
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2657.88 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 504.90 NGFR rabbit IgG(H+L) / donkey NGFR 261.89
A375-P7
E2
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2623.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 402.51 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 432.33
A375-P3
E3
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2609.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 401.06 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 4949.40
Colo858-P8
B3