Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 508.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.11 NGFR rabbit IgG(H+L) / donkey NGFR 738.14
WM115-P1
E7
WM-115 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 540.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.61 NGFR rabbit IgG(H+L) / donkey NGFR 908.83
WM115-P1
F10
WM-115 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 480.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.83 NGFR rabbit IgG(H+L) / donkey NGFR 889.64
WM115-P1
F11
WM-115 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 696.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.84 NGFR rabbit IgG(H+L) / donkey NGFR 1968.48
WM115-P1
F3
WM-115 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 548.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.08 NGFR rabbit IgG(H+L) / donkey NGFR 3060.55
WM115-P1
F4
WM-115 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 734.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 203.21 NGFR rabbit IgG(H+L) / donkey NGFR 624.02
WM115-P1
B10
WM-115 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 554.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 184.01 NGFR rabbit IgG(H+L) / donkey NGFR 580.80
WM115-P1
B11
WM-115 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1023.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 238.66 NGFR rabbit IgG(H+L) / donkey NGFR 1240.61
WM115-P1
B3
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 498.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.13 NGFR rabbit IgG(H+L) / donkey NGFR 2782.79
WM115-P1
B4
WM-115 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 684.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 192.63 NGFR rabbit IgG(H+L) / donkey NGFR 721.67
WM115-P1
B7
WM-115 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 768.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 194.55 NGFR rabbit IgG(H+L) / donkey NGFR 966.13
WM115-P1
B8
WM-115 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 521.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 144.36 NGFR rabbit IgG(H+L) / donkey NGFR 1045.50
WM115-P1
C10
WM-115 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 438.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.41 NGFR rabbit IgG(H+L) / donkey NGFR 1154.86
WM115-P1
C11
WM-115 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 433.75 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 142.86 NGFR rabbit IgG(H+L) / donkey NGFR 3068.52
WM115-P1
C5
WM-115 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 502.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.72 NGFR rabbit IgG(H+L) / donkey NGFR 1551.41
WM115-P1
C6
WM-115 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 473.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 129.87 NGFR rabbit IgG(H+L) / donkey NGFR 1998.34
WM115-P1
C8
WM-115 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 302.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.52 NGFR rabbit IgG(H+L) / donkey NGFR 1188.55
WM115-P1
C9
WM-115 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 643.96 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.77 NGFR rabbit IgG(H+L) / donkey NGFR 2353.22
WM115-P1
D2
WM-115 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 577.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.57 NGFR rabbit IgG(H+L) / donkey NGFR 2587.63
WM115-P1
D3
WM-115 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 338.96 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.06 NGFR rabbit IgG(H+L) / donkey NGFR 3192.24
WM115-P1
D5
WM-115 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 210.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 95.63 NGFR rabbit IgG(H+L) / donkey NGFR 2205.18
WM115-P1
D6
WM-115 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 641.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 176.08 NGFR rabbit IgG(H+L) / donkey NGFR 547.42
WM115-P1
E11
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1159.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 248.75 NGFR rabbit IgG(H+L) / donkey NGFR 1290.83
WM115-P1
E2
WM-115 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 388.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.90 NGFR rabbit IgG(H+L) / donkey NGFR 1241.39
WM115-P1
F9
WM-115 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 497.54 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 127.82 NGFR rabbit IgG(H+L) / donkey NGFR 1905.65
WM115-P1
F8