Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1339.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 236.98 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 267.54
Colo858-P9
B10
COLO 858 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 902.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 205.80 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 258.98
Colo858-P9
B11
COLO 858 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2512.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 349.85 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 142.66
Colo858-P9
B2
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2708.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 393.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 143.44
Colo858-P9
B3
COLO 858 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2225.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 329.43 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 197.64
Colo858-P9
B4
COLO 858 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2244.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 309.48 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 197.73
Colo858-P9
B5
COLO 858 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1578.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 242.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 212.87
Colo858-P9
B6
COLO 858 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 968.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 187.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 188.88
Colo858-P9
B7
COLO 858 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2426.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 354.27 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 226.49
Colo858-P9
B8
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2776.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 389.01 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 288.12
Colo858-P9
B9
COLO 858 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 167.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.73 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 657.31
Colo858-P9
C10
COLO 858 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 72.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 631.06
Colo858-P9
C11
COLO 858 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 828.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 231.09 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 648.00
Colo858-P9
C2
COLO 858 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 544.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 202.11 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 427.01
Colo858-P9
C3
COLO 858 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 620.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 222.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 484.31
Colo858-P9
C4
COLO 858 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 402.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 170.48 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 487.90
Colo858-P9
C5
COLO 858 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 237.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.43 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 699.34
Colo858-P9
C6
COLO 858 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 323.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 514.46
Colo858-P9
C7
COLO 858 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 413.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 234.38
Colo858-P9
C8
COLO 858 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 470.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.56 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 178.36
Colo858-P9
C9
COLO 858 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 124.94 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 815.68
Colo858-P9
D10
COLO 858 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 75.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 123.44 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 740.29
Colo858-P9
D11
COLO 858 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 654.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.86 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 681.06
Colo858-P9
D2
COLO 858 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 558.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 218.39 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 405.02
Colo858-P9
D3
COLO 858 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 439.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 208.10 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 562.52
Colo858-P9
D4