Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 607.30 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.59 NGFR rabbit IgG(H+L) / donkey NGFR 1208.85
Colo858-P1
F5
COLO 858 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 717.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 197.50 NGFR rabbit IgG(H+L) / donkey NGFR 163.65
Colo858-P1
F6
COLO 858 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 590.59 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.66 NGFR rabbit IgG(H+L) / donkey NGFR 90.87
Colo858-P1
F7
COLO 858 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 633.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 176.28 NGFR rabbit IgG(H+L) / donkey NGFR 258.47
Colo858-P1
F8
COLO 858 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 616.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 190.31 NGFR rabbit IgG(H+L) / donkey NGFR 104.72
Colo858-P1
F9
COLO 858 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 681.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 208.24 NGFR rabbit IgG(H+L) / donkey NGFR 48.54
Colo858-P1
G10
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 623.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 215.11 NGFR rabbit IgG(H+L) / donkey NGFR 47.15
Colo858-P1
G11
COLO 858 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 818.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 180.10 NGFR rabbit IgG(H+L) / donkey NGFR 774.72
Colo858-P1
G2
COLO 858 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 821.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 182.38 NGFR rabbit IgG(H+L) / donkey NGFR 846.07
Colo858-P1
G3
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 758.97 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 202.93 NGFR rabbit IgG(H+L) / donkey NGFR 1207.94
Colo858-P1
G4
COLO 858 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 690.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 176.22 NGFR rabbit IgG(H+L) / donkey NGFR 1039.96
Colo858-P1
G5
COLO 858 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 627.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 151.58 NGFR rabbit IgG(H+L) / donkey NGFR 225.14
Colo858-P1
G6
COLO 858 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 493.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 127.77 NGFR rabbit IgG(H+L) / donkey NGFR 149.72
Colo858-P1
G7
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 529.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.33 NGFR rabbit IgG(H+L) / donkey NGFR 402.57
Colo858-P1
G8
COLO 858 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 624.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.16 NGFR rabbit IgG(H+L) / donkey NGFR 128.02
Colo858-P1
G9
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1145.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 202.17 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 96.67
Colo858-P10
B10
COLO 858 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 693.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.13 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 99.11
Colo858-P10
B11
COLO 858 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2259.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 363.05 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 145.26
Colo858-P10
B2
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2470.40 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 370.68 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 125.42
Colo858-P10
B3
COLO 858 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1919.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 291.57 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 113.37
Colo858-P10
B4
COLO 858 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1941.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 278.32 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 104.06
Colo858-P10
B5
COLO 858 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1343.04 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 187.72 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 68.93
Colo858-P10
B6
COLO 858 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 843.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.92 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 82.52
Colo858-P10
B7
COLO 858 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2139.92 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 327.50 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 99.24
Colo858-P10
B8
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2327.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 344.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 109.17
Colo858-P10
B9