Viability and apoptosis measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of three compounds (viability/apoptosis dataset 2 of 2) - Dataset (ID:20273)

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Cell Small Molecule 1 Small Molecule 2 Small Molecule 3 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol 3 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Total Number of Cells (Nuclei) Number of Apoptotic Cells Relative Viability Mean Relative Viability Apoptosis Fraction Mean Apoptosis Fraction
WM-115 Vemurafenib Trametinib JNK-IN-8 3.1600 0.3160 1.0000
uM
 72
hr
 2 1666 918 0.1863 0.1847 0.5510 0.5321
WM-115 Vemurafenib Trametinib JNK-IN-8 3.1600 0.3160 1.0000
uM
 72
hr
 3 1441 729 0.1773 0.1847 0.5059 0.5321
WM-115 Vemurafenib Trametinib JNK-IN-8 3.1600 0.3160 3.1600
uM
 72
hr
 1 1182 926 0.0638 0.0736 0.7834 0.7638
WM-115 Vemurafenib Trametinib JNK-IN-8 3.1600 0.3160 3.1600
uM
 72
hr
 2 1148 850 0.0742 0.0736 0.7404 0.7638
WM-115 Vemurafenib Trametinib JNK-IN-8 3.1600 0.3160 3.1600
uM
 72
hr
 3 1432 1099 0.0829 0.0736 0.7675 0.7638
WM-115 Vemurafenib Trametinib PF562271 0.0000 0.0000 0.0000
uM
 72
hr
 1 4469 302 1.0191 1.0000 0.0676 0.0639
WM-115 Vemurafenib Trametinib PF562271 0.0000 0.0000 0.0000
uM
 72
hr
 2 4377 273 1.0037 1.0000 0.0624 0.0639
WM-115 Vemurafenib Trametinib PF562271 0.0000 0.0000 0.0000
uM
 72
hr
 3 4259 263 0.9773 1.0000 0.0618 0.0639
WM-115 Vemurafenib Trametinib PF562271 0.0000 0.0000 0.3160
uM
 72
hr
 1 3656 272 0.8276 0.7973 0.0744 0.0750
WM-115 Vemurafenib Trametinib PF562271 0.0000 0.0000 0.3160
uM
 72
hr
 2 3452 304 0.7699 0.7973 0.0881 0.0750
WM-115 Vemurafenib Trametinib PF562271 0.0000 0.0000 0.3160
uM
 72
hr
 3 3466 217 0.7946 0.7973 0.0626 0.0750
WM-115 Vemurafenib Trametinib PF562271 0.0000 0.0000 1.0000
uM
 72
hr
 1 2821 227 0.6344 0.5869 0.0805 0.0856
WM-115 Vemurafenib Trametinib PF562271 0.0000 0.0000 1.0000
uM
 72
hr
 2 2513 234 0.5573 0.5869 0.0931 0.0856
WM-115 Vemurafenib Trametinib PF562271 0.0000 0.0000 1.0000
uM
 72
hr
 3 2538 211 0.5691 0.5869 0.0831 0.0856
WM-115 Vemurafenib Trametinib PF562271 0.0000 0.0000 3.1600
uM
 72
hr
 1 1914 355 0.3813 0.3710 0.1855 0.1613
WM-115 Vemurafenib Trametinib PF562271 0.0000 0.0000 3.1600
uM
 72
hr
 2 1797 271 0.3732 0.3710 0.1508 0.1613
WM-115 Vemurafenib Trametinib PF562271 0.0000 0.0000 3.1600
uM
 72
hr
 3 1720 254 0.3585 0.3710 0.1477 0.1613
WM-115 Vemurafenib Trametinib PF562271 0.1000 0.0100 0.0000
uM
 72
hr
 1 1923 534 0.3397 0.3532 0.2777 0.2765
WM-115 Vemurafenib Trametinib PF562271 0.1000 0.0100 0.0000
uM
 72
hr
 2 2058 545 0.3700 0.3532 0.2648 0.2765
WM-115 Vemurafenib Trametinib PF562271 0.1000 0.0100 0.0000
uM
 72
hr
 3 2007 576 0.3500 0.3532 0.2870 0.2765
WM-115 Vemurafenib Trametinib PF562271 0.1000 0.0100 0.3160
uM
 72
hr
 1 1634 576 0.2587 0.2662 0.3525 0.3525
WM-115 Vemurafenib Trametinib PF562271 0.1000 0.0100 0.3160
uM
 72
hr
 2 1718 620 0.2685 0.2662 0.3609 0.3525
WM-115 Vemurafenib Trametinib PF562271 0.1000 0.0100 0.3160
uM
 72
hr
 3 1691 582 0.2712 0.2662 0.3442 0.3525
WM-115 Vemurafenib Trametinib PF562271 0.1000 0.0100 1.0000
uM
 72
hr
 1 1487 536 0.2326 0.2360 0.3605 0.3345
WM-115 Vemurafenib Trametinib PF562271 0.1000 0.0100 1.0000
uM
 72
hr
 2 1455 482 0.2380 0.2360 0.3313 0.3345