Viability and apoptosis measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of three compounds (viability/apoptosis dataset 2 of 2) - Dataset (ID:20273)

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Cell Small Molecule 1 Small Molecule 2 Small Molecule 3 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol 3 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Total Number of Cells (Nuclei) Number of Apoptotic Cells Relative Viability Mean Relative Viability Apoptosis Fraction Mean Apoptosis Fraction
A-375 Vemurafenib Trametinib PF562271 0.0000 0.0000 0.0000
uM
 72
hr
 2 21851 1210 1.0372 1.0000 0.0554 0.0502
A-375 Vemurafenib Trametinib PF562271 0.0000 0.0000 0.0000
uM
 72
hr
 1 21287 1027 1.0181 1.0000 0.0482 0.0502
A-375 Vemurafenib Trametinib PF562271 0.0000 0.0000 0.3160
uM
 72
hr
 3 20259 1054 0.9651 0.9140 0.0520 0.0504
A-375 Vemurafenib Trametinib PF562271 0.0000 0.0000 0.0000
uM
 72
hr
 3 19729 929 0.9447 1.0000 0.0471 0.0502
A-375 Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 0.3160
uM
 72
hr
 3 19598 815 1.1074 1.0240 0.0416 0.0404
A-375 Vemurafenib Trametinib PF562271 0.0000 0.0000 0.3160
uM
 72
hr
 2 19593 1032 0.9327 0.9140 0.0527 0.0504
A-375 Vemurafenib Trametinib Defactinib 0.0000 0.0000 0.0000
uM
 72
hr
 2 19509 973 1.0489 1.0000 0.0499 0.0517
A-375 Vemurafenib Trametinib Defactinib 0.0000 0.0000 0.0000
uM
 72
hr
 1 19308 1026 1.0346 1.0000 0.0531 0.0517
A-375 Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 0.3160
uM
 72
hr
 2 18988 841 1.0699 1.0240 0.0443 0.0404
A-375 Vemurafenib Trametinib Saracatinib 0.0000 0.0000 0.0000
uM
 72
hr
 1 18392 833 1.0430 1.0000 0.0453 0.0438
A-375 Vemurafenib Trametinib Saracatinib 0.0000 0.0000 0.0000
uM
 72
hr
 2 18289 776 1.0403 1.0000 0.0424 0.0438
A-375 Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 0.0000
uM
 72
hr
 2 18030 727 1.0201 1.0000 0.0403 0.0484
A-375 Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 0.0000
uM
 72
hr
 3 17907 936 1.0006 1.0000 0.0523 0.0484
A-375 Vemurafenib Trametinib PF562271 0.0000 0.0000 0.3160
uM
 72
hr
 1 17620 818 0.8443 0.9140 0.0464 0.0504
A-375 Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 0.0000
uM
 72
hr
 1 17534 924 0.9793 1.0000 0.0527 0.0484
A-375 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.0000
uM
 72
hr
 3 17422 897 1.0619 1.0000 0.0515 0.0508
A-375 Vemurafenib Trametinib Defactinib 0.0000 0.0000 0.0000
uM
 72
hr
 3 17086 890 0.9165 1.0000 0.0521 0.0517
A-375 Vemurafenib Trametinib Defactinib 0.0000 0.0000 0.3160
uM
 72
hr
 3 16548 819 0.8901 0.8521 0.0495 0.0546
A-375 Vemurafenib Trametinib I-BET151 0.0000 0.0000 0.0000
uM
 72
hr
 2 16450 1693 1.0246 1.0000 0.1029 0.1003
A-375 Vemurafenib Trametinib I-BET151 0.0000 0.0000 0.0000
uM
 72
hr
 3 16303 1628 1.0189 1.0000 0.0999 0.1003
A-375 Vemurafenib Trametinib Defactinib 0.0000 0.0000 0.3160
uM
 72
hr
 2 16146 916 0.8618 0.8521 0.0567 0.0546
A-375 Vemurafenib Trametinib Saracatinib 0.0000 0.0000 0.0000
uM
 72
hr
 3 16139 705 0.9168 1.0000 0.0437 0.0438
A-375 Vemurafenib Trametinib I-BET 0.0000 0.0000 0.0000
uM
 72
hr
 2 16108 728 1.1037 1.0000 0.0452 0.0842
A-375 Vemurafenib Trametinib Saracatinib 0.0000 0.0000 0.3160
uM
 72
hr
 3 16012 776 0.9050 0.8466 0.0485 0.0510
A-375 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.0000
uM
 72
hr
 2 15978 849 0.9722 1.0000 0.0531 0.0508
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