Viability and apoptosis measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of three compounds (viability/apoptosis dataset 2 of 2) - Dataset (ID:20273)

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Cell Small Molecule 1 Small Molecule 2 Small Molecule 3 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol 3 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Total Number of Cells (Nuclei) Number of Apoptotic Cells Relative Viability Mean Relative Viability Apoptosis Fraction Mean Apoptosis Fraction
MMAC-SF Vemurafenib Trametinib PF562271 1.0000 0.1000 0.3160
uM
 72
hr
 2 158 157 0.0009 0.1712 0.9937 0.5833
MMAC-SF Vemurafenib Trametinib PF562271 3.1600 0.3160 3.1600
uM
 72
hr
 3 186 87 0.0856 0.1014 0.4677 0.4221
MMAC-SF Vemurafenib Trametinib Defactinib 1.0000 0.1000 3.1600
uM
 72
hr
 3 195 113 0.0792 0.1361 0.5795 0.5198
MMAC-SF Vemurafenib Trametinib Defactinib 3.1600 0.3160 3.1600
uM
 72
hr
 3 198 83 0.1110 0.1361 0.4192 0.3969
MMAC-SF Vemurafenib Trametinib PF562271 3.1600 0.3160 3.1600
uM
 72
hr
 2 203 82 0.1046 0.1014 0.4039 0.4221
MMAC-SF Vemurafenib Trametinib PF562271 0.3160 0.0316 3.1600
uM
 72
hr
 3 203 100 0.0890 0.1329 0.4926 0.4906
MMAC-SF Vemurafenib Trametinib PF562271 1.0000 0.1000 3.1600
uM
 72
hr
 3 206 96 0.0951 0.1236 0.4660 0.4924
MMAC-SF Vemurafenib Trametinib Defactinib 3.1600 0.3160 3.1600
uM
 72
hr
 2 218 79 0.1342 0.1361 0.3624 0.3969
MMAC-SF Vemurafenib Trametinib PF562271 3.1600 0.3160 3.1600
uM
 72
hr
 1 218 86 0.1141 0.1014 0.3945 0.4221
MMAC-SF Vemurafenib Trametinib PF562271 3.1600 0.3160 1.0000
uM
 72
hr
 3 221 95 0.1089 0.1628 0.4299 0.4419
MMAC-SF Vemurafenib Trametinib Defactinib 3.1600 0.3160 1.0000
uM
 72
hr
 3 223 93 0.1255 0.1986 0.4170 0.4158
MMAC-SF Vemurafenib Trametinib Defactinib 0.3160 0.0316 1.0000
uM
 72
hr
 3 235 134 0.0975 0.1699 0.5702 0.5080
MMAC-SF Vemurafenib Trametinib Saracatinib 3.1600 0.3160 3.1600
uM
 72
hr
 3 241 82 0.1574 0.2033 0.3402 0.3455
MMAC-SF Vemurafenib Trametinib Saracatinib 3.1600 0.3160 1.0000
uM
 72
hr
 3 244 95 0.1475 0.2512 0.3893 0.3722
MMAC-SF Vemurafenib Trametinib Defactinib 0.1000 0.0100 3.1600
uM
 72
hr
 3 244 153 0.0879 0.1329 0.6270 0.6191
MMAC-SF Vemurafenib Trametinib Defactinib 0.3160 0.0316 3.1600
uM
 72
hr
 3 245 157 0.0850 0.1310 0.6408 0.6198
MMAC-SF Vemurafenib Trametinib PF562271 1.0000 0.1000 1.0000
uM
 72
hr
 3 265 119 0.1262 0.1784 0.4491 0.4506
MMAC-SF Vemurafenib Trametinib Defactinib 3.1600 0.3160 0.3160
uM
 72
hr
 2 268 113 0.1497 0.1625 0.4216 0.4050
MMAC-SF Vemurafenib Trametinib JNK-IN-8 3.1600 0.3160 1.0000
uM
 72
hr
 3 272 104 0.1663 0.2710 0.3824 0.3368
MZ7-mel Vemurafenib Trametinib Dasatinib 3.1600 0.3160 3.1600
uM
 72
hr
 3 274 57 0.0651 0.0797 0.2080 0.2019
MMAC-SF Vemurafenib Trametinib Defactinib 1.0000 0.1000 1.0000
uM
 72
hr
 3 276 137 0.1342 0.2053 0.4964 0.4547
MMAC-SF Vemurafenib Trametinib Defactinib 3.1600 0.3160 0.3160
uM
 72
hr
 3 286 116 0.1641 0.1625 0.4056 0.4050
MMAC-SF Vemurafenib Trametinib Defactinib 3.1600 0.3160 3.1600
uM
 72
hr
 1 286 117 0.1632 0.1361 0.4091 0.3969
MMAC-SF Vemurafenib Trametinib Saracatinib 3.1600 0.3160 3.1600
uM
 72
hr
 2 289 92 0.1950 0.2033 0.3183 0.3455
MMAC-SF Vemurafenib Trametinib Defactinib 1.0000 0.1000 3.1600
uM
 72
hr
 2 292 144 0.1429 0.1361 0.4932 0.5198
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