Viability and apoptosis measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of three compounds (viability/apoptosis dataset 2 of 2) - Dataset (ID:20273)

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Cell Small Molecule 1 Small Molecule 2 Small Molecule 3 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol 3 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Total Number of Cells (Nuclei) Number of Apoptotic Cells Relative Viability Mean Relative Viability Apoptosis Fraction Mean Apoptosis Fraction
A-375 Vemurafenib Trametinib I-BET151 0.0000 0.0000 0.0000
uM
 72
hr
 2 16450 1693 1.0246 1.0000 0.1029 0.1003
COLO 858 Vemurafenib Trametinib Defactinib 3.1600 0.3160 1.0000
uM
 72
hr
 1 1719 1644 0.0106 0.0087 0.9564 0.9527
COLO 858 Vemurafenib Trametinib Defactinib 3.1600 0.3160 0.0000
uM
 72
hr
 3 1724 1632 0.0129 0.0136 0.9466 0.9358
A-375 Vemurafenib Trametinib I-BET151 0.0000 0.0000 0.0000
uM
 72
hr
 3 16303 1628 1.0189 1.0000 0.0999 0.1003
A-375 Vemurafenib Trametinib Belinostat 0.0000 0.0000 0.0000
uM
 72
hr
 3 15897 1615 1.0798 1.0000 0.1016 0.1003
COLO 858 Vemurafenib Trametinib Defactinib 3.1600 0.3160 0.0000
uM
 72
hr
 2 1636 1567 0.0097 0.0136 0.9578 0.9358
COLO 858 Vemurafenib Trametinib Dasatinib 3.1600 0.3160 0.0000
uM
 72
hr
 3 1665 1566 0.0164 0.0156 0.9405 0.9275
A-375 Vemurafenib Trametinib I-BET 0.0000 0.0000 0.0000
uM
 72
hr
 1 15279 1529 0.9867 1.0000 0.1001 0.0842
A-375 Vemurafenib Trametinib I-BET 0.0000 0.0000 0.0000
uM
 72
hr
 3 14201 1526 0.9096 1.0000 0.1075 0.0842
A-375 Vemurafenib Trametinib Belinostat 0.0000 0.0000 0.0000
uM
 72
hr
 2 15480 1512 1.0561 1.0000 0.0977 0.1003
A-375 Vemurafenib Trametinib (+)-JQ1 0.0000 0.0000 0.0000
uM
 72
hr
 2 14936 1503 0.9820 1.0000 0.1006 0.0658
A-375 Vemurafenib Trametinib I-BET151 0.0000 0.0000 0.0000
uM
 72
hr
 1 15278 1501 0.9565 1.0000 0.0982 0.1003
COLO 858 Vemurafenib Trametinib Saracatinib 3.1600 0.3160 0.0000
uM
 72
hr
 3 1504 1422 0.0137 0.0124 0.9455 0.9350
COLO 858 Vemurafenib Trametinib Defactinib 1.0000 0.1000 1.0000
uM
 72
hr
 1 1549 1404 0.0204 0.0153 0.9064 0.9147
COLO 858 Vemurafenib Trametinib Defactinib 0.0000 0.0000 0.3160
uM
 72
hr
 2 7902 1360 0.9208 0.9286 0.1721 0.1630
COLO 858 Vemurafenib Trametinib Dasatinib 3.1600 0.3160 1.0000
uM
 72
hr
 1 1387 1358 0.0048 0.0049 0.9791 0.9743
COLO 858 Vemurafenib Trametinib Defactinib 0.0000 0.0000 0.0000
uM
 72
hr
 2 9127 1356 1.0938 1.0000 0.1486 0.1477
COLO 858 Vemurafenib Trametinib JNK-IN-8 3.1600 0.3160 1.0000
uM
 72
hr
 2 1414 1345 0.0104 0.0226 0.9512 0.8932
COLO 858 Vemurafenib Trametinib Defactinib 0.0000 0.0000 0.3160
uM
 72
hr
 3 8236 1331 0.9719 0.9286 0.1616 0.1630
COLO 858 Vemurafenib Trametinib JNK-IN-8 3.1600 0.3160 0.0000
uM
 72
hr
 2 1412 1324 0.0133 0.0186 0.9377 0.9092
COLO 858 Vemurafenib Trametinib Saracatinib 3.1600 0.3160 1.0000
uM
 72
hr
 1 1361 1317 0.0074 0.0051 0.9677 0.9739
COLO 858 Vemurafenib Trametinib Defactinib 1.0000 0.1000 0.0000
uM
 72
hr
 3 1706 1317 0.0548 0.0458 0.7720 0.7801
A-375 Vemurafenib Trametinib Belinostat 0.0000 0.0000 0.0000
uM
 72
hr
 1 12722 1293 0.8641 1.0000 0.1016 0.1003
COLO 858 Vemurafenib Trametinib Defactinib 0.0000 0.0000 0.0000
uM
 72
hr
 1 8737 1282 1.0493 1.0000 0.1467 0.1477
COLO 858 Vemurafenib Trametinib Defactinib 0.3160 0.0316 1.0000
uM
 72
hr
 1 1504 1272 0.0327 0.0247 0.8457 0.8594
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