Viability and apoptosis measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of three compounds (viability/apoptosis dataset 2 of 2) - Dataset (ID:20273)

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Cell Small Molecule 1 Small Molecule 2 Small Molecule 3 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol 3 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Total Number of Cells (Nuclei) Number of Apoptotic Cells Relative Viability Mean Relative Viability Apoptosis Fraction Mean Apoptosis Fraction
WM-115 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.0000
uM
 72
hr
 3 2384 556 0.4286 0.4016 0.2332 0.2480
WM-115 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 3.1600
uM
 72
hr
 2 1153 685 0.1097 0.1154 0.5941 0.5851
WM-115 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 1.0000
uM
 72
hr
 1 2644 476 0.5083 0.5260 0.1800 0.1772
WM-115 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.0000
uM
 72
hr
 2 2289 595 0.3972 0.4016 0.2599 0.2480
WM-115 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 1.0000
uM
 72
hr
 1 1238 740 0.1168 0.1322 0.5977 0.5586
WM-115 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 3.1600
uM
 72
hr
 1 1219 713 0.1186 0.1154 0.5849 0.5851
WM-115 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.0000
uM
 72
hr
 3 4625 298 1.0145 1.0000 0.0644 0.0618
WM-115 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.3160
uM
 72
hr
 3 2741 592 0.5039 0.5131 0.2160 0.2141
WM-115 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 3.1600
uM
 72
hr
 1 2254 446 0.4239 0.4392 0.1979 0.1684
WM-115 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.0000
uM
 72
hr
 1 2157 541 0.3789 0.4016 0.2508 0.2480
WM-115 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.3160
uM
 72
hr
 2 1302 783 0.1217 0.1195 0.6014 0.6100
WM-115 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.3160
uM
 72
hr
 3 1259 734 0.1231 0.1195 0.5830 0.6100
WM-115 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 1.0000
uM
 72
hr
 2 1344 776 0.1332 0.1322 0.5774 0.5586
WM-115 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 1.0000
uM
 72
hr
 3 1252 627 0.1465 0.1322 0.5008 0.5586
WM-115 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.0000
uM
 72
hr
 1 4353 259 0.9599 1.0000 0.0595 0.0618
WM-115 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.0000
uM
 72
hr
 2 4661 287 1.0256 1.0000 0.0616 0.0618
WM-115 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.3160
uM
 72
hr
 1 2739 569 0.5088 0.5131 0.2077 0.2141
WM-115 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.3160
uM
 72
hr
 2 2874 628 0.5266 0.5131 0.2185 0.2141
WM-115 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 1.0000
uM
 72
hr
 2 2691 483 0.5177 0.5260 0.1795 0.1772
WM-115 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 1.0000
uM
 72
hr
 3 2843 489 0.5519 0.5260 0.1720 0.1772
WM-115 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 3.1600
uM
 72
hr
 2 2129 338 0.4199 0.4392 0.1588 0.1684
WM-115 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 3.1600
uM
 72
hr
 3 2374 353 0.4739 0.4392 0.1487 0.1684
WM-115 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.3160
uM
 72
hr
 1 1368 883 0.1137 0.1195 0.6455 0.6100
WM-115 Vemurafenib Trametinib Dasatinib 0.3160 0.0316 0.0000
uM
 72
hr
 1 2058 623 0.3365 0.3340 0.3027 0.3130
WM-115 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 3.1600
uM
 72
hr
 3 1187 684 0.1179 0.1154 0.5762 0.5851
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