Viability and apoptosis measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of three compounds (viability/apoptosis dataset 2 of 2) - Dataset (ID:20273)

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Cell Small Molecule 1 Small Molecule 2 Small Molecule 3 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol 3 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Total Number of Cells (Nuclei) Number of Apoptotic Cells Relative Viability Mean Relative Viability Apoptosis Fraction Mean Apoptosis Fraction
WM-115 Vemurafenib Trametinib Saracatinib 0.0000 0.0000 3.1600
uM
 72
hr
 2 2965 234 0.7208 0.7205 0.0789 0.0832
WM-115 Vemurafenib Trametinib Saracatinib 0.0000 0.0000 3.1600
uM
 72
hr
 3 2722 220 0.6603 0.7205 0.0808 0.0832
WM-115 Vemurafenib Trametinib Saracatinib 0.1000 0.0100 0.0000
uM
 72
hr
 1 1912 534 0.3637 0.3651 0.2793 0.3112
WM-115 Vemurafenib Trametinib Saracatinib 0.1000 0.0100 0.0000
uM
 72
hr
 2 1985 683 0.3436 0.3651 0.3441 0.3112
WM-115 Vemurafenib Trametinib Saracatinib 0.1000 0.0100 0.0000
uM
 72
hr
 3 2131 661 0.3880 0.3651 0.3102 0.3112
WM-115 Vemurafenib Trametinib Saracatinib 0.1000 0.0100 0.3160
uM
 72
hr
 1 1903 796 0.2922 0.3055 0.4183 0.3977
WM-115 Vemurafenib Trametinib Saracatinib 0.1000 0.0100 0.3160
uM
 72
hr
 2 1890 767 0.2964 0.3055 0.4058 0.3977
WM-115 Vemurafenib Trametinib Saracatinib 0.1000 0.0100 0.3160
uM
 72
hr
 3 1970 727 0.3281 0.3055 0.3690 0.3977
WM-115 Vemurafenib Trametinib Saracatinib 0.1000 0.0100 1.0000
uM
 72
hr
 1 1752 668 0.2861 0.2723 0.3813 0.3965
WM-115 Vemurafenib Trametinib Saracatinib 0.1000 0.0100 1.0000
uM
 72
hr
 2 1877 822 0.2784 0.2723 0.4379 0.3965
WM-115 Vemurafenib Trametinib Saracatinib 0.1000 0.0100 1.0000
uM
 72
hr
 3 1518 562 0.2523 0.2723 0.3702 0.3965
WM-115 Vemurafenib Trametinib Saracatinib 0.1000 0.0100 3.1600
uM
 72
hr
 1 1647 700 0.2499 0.2290 0.4250 0.4257
WM-115 Vemurafenib Trametinib Saracatinib 0.1000 0.0100 3.1600
uM
 72
hr
 2 1550 676 0.2307 0.2290 0.4361 0.4257
WM-115 Vemurafenib Trametinib Saracatinib 0.1000 0.0100 3.1600
uM
 72
hr
 3 1339 557 0.2064 0.2290 0.4160 0.4257
WM-115 Vemurafenib Trametinib Saracatinib 0.3160 0.0316 0.0000
uM
 72
hr
 1 1600 583 0.2684 0.3049 0.3644 0.3755
WM-115 Vemurafenib Trametinib Saracatinib 0.3160 0.0316 0.0000
uM
 72
hr
 2 1987 748 0.3270 0.3049 0.3764 0.3755
WM-115 Vemurafenib Trametinib Saracatinib 0.3160 0.0316 0.0000
uM
 72
hr
 3 1970 760 0.3193 0.3049 0.3858 0.3755
WM-115 Vemurafenib Trametinib Saracatinib 0.3160 0.0316 0.3160
uM
 72
hr
 1 1722 846 0.2312 0.2416 0.4913 0.4746
WM-115 Vemurafenib Trametinib Saracatinib 0.3160 0.0316 0.3160
uM
 72
hr
 2 1756 806 0.2507 0.2416 0.4590 0.4746
WM-115 Vemurafenib Trametinib Saracatinib 0.3160 0.0316 0.3160
uM
 72
hr
 3 1747 827 0.2428 0.2416 0.4734 0.4746
WM-115 Vemurafenib Trametinib Saracatinib 0.3160 0.0316 1.0000
uM
 72
hr
 1 1749 887 0.2275 0.2108 0.5071 0.4999
WM-115 Vemurafenib Trametinib Saracatinib 0.3160 0.0316 1.0000
uM
 72
hr
 2 1583 777 0.2127 0.2108 0.4908 0.4999
WM-115 Vemurafenib Trametinib Saracatinib 0.3160 0.0316 1.0000
uM
 72
hr
 3 1461 733 0.1921 0.2108 0.5017 0.4999
WM-115 Vemurafenib Trametinib Saracatinib 0.3160 0.0316 3.1600
uM
 72
hr
 1 1620 904 0.1890 0.1786 0.5580 0.5384
WM-115 Vemurafenib Trametinib Saracatinib 0.3160 0.0316 3.1600
uM
 72
hr
 2 1445 784 0.1745 0.1786 0.5426 0.5384