Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 378.8 6
0191-0352
 377.14 351.79 219 57.70 0.827
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 347.8 6
0192-0351
 498.07 351.01 213 53.94 0.920
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 33064.1 6
0193-0353
 692.35 352.74 324 67.36 0.897
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 402.0 6
0194-0357
 364.46 357.85 224 56.18 0.892
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 467.0 6
0195-0360
 461.11 360.46 289 67.84 0.789
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 435.9 6
0196-0361
 649.92 360.91 230 56.53 0.905
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 350.2 6
0197-0363
 727.33 363.16 361 72.08 0.873
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 453.5 6
0198-0362
 812.22 361.84 247 58.53 0.906
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 489.1 6
0199-0362
 324.84 362.43 285 62.77 0.909
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 308.5 6
0200-0364
 433.33 364.31 205 53.36 0.905
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 485.9 6
0201-0369
 678.26 369.38 217 56.77 0.846
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 383.9 6
0202-0376
 638.40 376.92 207 56.18 0.824
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 213.5 6
0203-0372
 911.90 372.84 164 47.21 0.925
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 244.3 6
0204-0379
 717.08 380.18 358 71.26 0.886
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 262.0 6
0205-0383
 151.22 382.57 305 66.18 0.875
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 446.7 6
0206-0382
 289.74 381.60 226 57.36 0.863
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 326.9 6
0207-0386
 890.49 386.72 261 61.84 0.858
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 311.7 6
0208-0387
 834.65 386.96 213 56.87 0.828
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 287.1 6
0209-0390
 82.98 390.33 240 58.77 0.873
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 285.5 6
0210-0392
 36.05 392.71 211 67.01 0.590
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 396.2 6
0211-0392
 183.93 392.40 256 60.53 0.878
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 510.9 6
0212-0392
 298.98 391.86 144 51.01 0.695
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 241.4 6
0213-0394
 114.07 394.56 296 64.43 0.896
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 307.4 6
0214-0394
 281.33 394.54 232 57.11 0.894
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 398.3 6
0215-0394
 412.94 394.12 236 58.18 0.876