Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 1463.5 8
0026-0098
 431.25 98.10 437 83.15 0.794
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 261.4 8
0027-0100
 366.00 100.19 409 76.67 0.874
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 372.7 8
0028-0110
 622.43 110.49 865 119.98 0.755
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 247.3 8
0029-0107
 652.75 107.06 446 81.01 0.854
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 197.2 8
0030-0111
 703.55 110.95 404 76.43 0.869
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 213.1 8
0031-0113
 73.92 113.83 409 77.84 0.848
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 784.8 8
0032-0114
 142.14 114.63 404 78.91 0.815
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 554.9 8
0033-0116
 682.50 115.95 406 75.36 0.898
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 486.8 8
0034-0119
 820.27 119.15 422 77.26 0.889
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 281.4 8
0035-0132
 728.98 132.30 480 86.57 0.805
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 204.5 8
0036-0131
 552.04 130.76 407 76.67 0.870
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 356.8 8
0037-0141
 469.46 141.36 908 135.68 0.620
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 324.3 8
0038-0132
 614.30 132.68 390 79.36 0.778
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 1745.5 8
0039-0144
 279.56 144.71 608 95.64 0.835
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 265.9 8
0040-0143
 414.75 143.33 406 77.50 0.849
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 641.9 8
0041-0154
 61.26 154.33 437 80.33 0.851
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 253.8 8
0042-0153
 869.81 153.09 405 77.26 0.853
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 235.0 8
0043-0157
 138.42 157.09 429 82.08 0.800
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 277.6 8
0044-0157
 562.84 158.13 412 80.33 0.802
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 1110.8 8
0045-0159
 165.70 159.40 498 90.81 0.759
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 250.6 8
0046-0165
 521.33 164.82 413 76.67 0.883
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 240.4 8
0047-0167
 739.78 167.56 428 78.08 0.882
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 471.9 8
0048-0168
 624.74 168.55 454 80.77 0.875
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 304.4 8
0049-0183
 567.41 184.60 849 121.78 0.719
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 278.9 8
0050-0183
 384.62 183.38 418 79.50 0.831