Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 264.7 4
0269-0545
612.72 545.77 302 66.67 0.854
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 334.4 4
0270-0546
483.70 546.75 285 67.60 0.784
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 288.0 4
0271-0546
120.03 546.11 297 64.18 0.906
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 329.7 4
0272-0551
386.36 551.34 382 75.50 0.842
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 286.2 4
0273-0545
452.01 545.29 113 39.21 0.923
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 470.7 4
0274-0549
826.28 549.64 208 55.94 0.835
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 359.8 4
0275-0553
769.54 553.39 208 57.01 0.804
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 316.8 4
0276-0553
896.29 553.02 252 59.60 0.892
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 302.6 4
0277-0556
169.22 558.38 335 78.18 0.689
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 457.1 4
0278-0552
257.75 552.29 134 46.04 0.794
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 305.4 4
0279-0555
927.66 554.65 218 53.70 0.950
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 293.9 4
0280-0562
695.36 561.59 340 69.84 0.876
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 318.5 4
0281-0560
39.92 559.67 217 54.28 0.925
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 305.3 4
0282-0559
792.50 558.57 225 55.94 0.904
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 267.5 4
0283-0562
661.76 562.49 244 58.53 0.895
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 307.3 4
0284-0566
442.11 565.66 250 59.11 0.899
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 591.1 4
0285-0571
626.34 571.54 350 72.91 0.827
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 641.2 4
0286-0573
745.64 573.60 268 64.28 0.815
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 435.0 4
0287-0579
202.08 578.90 254 59.60 0.899
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 330.8 4
0288-0580
355.71 580.32 261 71.26 0.646
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 350.6 4
0289-0578
55.91 579.20 240 59.11 0.863
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 464.0 4
0290-0584
101.22 583.64 271 62.53 0.871
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 411.5 4
0291-0587
896.46 587.20 239 57.94 0.895
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 317.1 4
0292-0590
503.77 590.15 227 55.70 0.919
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 214.0 4
0293-0593
414.11 594.58 329 71.01 0.820