Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 2031.2 7
0129-0289
 160.03 289.59 504 84.43 0.889
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 2684.1 7
0130-0286
 717.44 286.35 251 59.94 0.878
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 500.8 7
0131-0285
 226.22 284.88 147 47.46 0.820
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 2121.4 7
0132-0287
 884.60 287.66 261 63.01 0.826
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 449.8 7
0133-0287
 266.28 286.81 204 54.28 0.870
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 1895.4 7
0134-0295
 42.73 293.97 470 84.67 0.824
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 571.6 7
0135-0290
 409.83 289.56 218 55.11 0.902
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 1613.5 7
0136-0296
 638.34 296.98 357 70.43 0.904
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 3218.7 7
0137-0296
 95.19 296.13 243 59.36 0.867
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 2018.0 7
0138-0300
 300.90 299.53 352 70.18 0.898
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 2461.8 7
0139-0299
 441.91 299.04 285 62.53 0.916
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 2062.7 7
0140-0303
 747.06 302.21 284 64.77 0.851
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 957.3 7
0141-0300
 226.96 300.39 100 38.04 0.868
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 273.3 7
0142-0307
 921.16 307.62 285 79.50 0.567
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 1813.4 7
0143-0304
 356.23 304.27 278 65.01 0.827
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 1745.2 7
0144-0303
 621.62 304.04 199 53.11 0.886
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 1944.0 7
0145-0309
 250.39 309.05 279 69.01 0.736
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 1502.7 7
0146-0312
 495.72 311.78 412 83.98 0.734
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 1963.3 7
0147-0310
 729.52 310.80 232 57.94 0.868
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 2564.4 7
0148-0311
 762.11 310.96 229 57.11 0.882
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 2355.4 7
0149-0310
 528.20 310.63 234 58.28 0.866
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 2959.7 7
0150-0312
 795.82 311.91 291 64.18 0.888
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 724.7 7
0151-0316
 235.32 316.79 240 70.43 0.608
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 1816.7 7
0152-0312
 410.34 312.51 248 58.63 0.907
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-2
 Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 Rb (pS807; pS811) 2315.7 7
0153-0317
 371.38 316.56 293 65.94 0.847