Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 485.1 8
0055-0621
 146.69 622.02 198 57.11 0.763
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 419.6 8
0056-0621
 290.70 621.09 249 60.18 0.864
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 391.2 8
0057-0639
 577.61 640.06 225 62.53 0.723
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 426.6 8
0058-0657
 135.73 657.10 210 61.70 0.693
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 343.1 8
0059-0652
 674.40 652.07 277 69.36 0.724
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 535.2 8
0060-0666
 415.93 666.00 171 55.11 0.707
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 503.7 8
0061-0699
 370.97 699.10 183 51.70 0.860
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 398.2 8
0062-0701
 133.33 701.42 195 51.70 0.917
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 124.0 8
0063-0709
 842.07 709.27 198 51.70 0.931
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 261.6 9
0001-0034
 31.77 34.33 273 65.01 0.812
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 451.5 9
0002-0034
 411.40 35.09 286 64.77 0.857
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 298.8 9
0003-0040
 489.21 39.94 280 65.84 0.812
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 330.1 9
0004-0049
 727.83 49.69 258 61.11 0.868
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 10125.3 9
0005-0071
 43.56 71.40 286 64.28 0.870
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 643.3 9
0006-0103
 476.99 103.18 279 61.36 0.931
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 376.1 9
0007-0106
 354.74 106.26 246 59.60 0.870
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 466.3 9
0008-0118
 570.68 118.15 240 60.53 0.823
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 393.4 9
0009-0145
 347.01 145.15 199 53.11 0.886
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 605.2 9
0010-0148
 694.70 149.05 194 59.36 0.692
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 329.9 9
0011-0150
 125.25 150.20 225 59.46 0.800
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 357.2 9
0012-0157
 97.26 157.47 266 59.11 0.957
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 319.5 9
0013-0183
 323.97 183.54 246 61.60 0.815
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 401.3 9
0014-0194
 251.35 194.46 224 57.36 0.856
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 295.1 9
0015-0206
 858.14 205.90 261 62.18 0.848
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 364.2 9
0016-0210
 14.17 209.59 216 55.60 0.878