Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 274.9 9
0064-0333
831.25 333.40 333 69.26 0.872
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 261.6 9
0065-0341
545.88 341.42 289 63.36 0.905
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 288.1 9
0066-0341
871.51 341.54 301 64.43 0.911
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 205.7 9
0067-0346
43.39 345.92 356 69.01 0.939
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 465.8 9
0068-0350
225.43 350.33 358 73.26 0.838
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 346.2 9
0069-0364
425.99 364.39 291 69.36 0.760
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 300.7 9
0070-0363
784.03 363.41 333 68.77 0.885
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 229.4 9
0071-0368
75.81 368.43 329 66.77 0.927
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 288.0 9
0072-0370
728.14 370.19 368 71.84 0.896
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 266.8 9
0073-0377
675.67 377.52 431 94.81 0.603
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 299.1 9
0074-0384
562.15 380.29 405 96.47 0.547
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 239.7 9
0075-0380
839.73 380.27 381 72.43 0.913
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 250.3 9
0076-0395
607.01 394.57 312 70.67 0.785
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 309.1 9
0077-0401
333.46 400.98 249 60.53 0.854
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 303.6 9
0078-0401
429.53 401.57 283 62.43 0.913
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 323.6 9
0079-0413
477.40 413.11 239 58.53 0.877
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 278.6 9
0080-0418
752.22 417.97 326 67.94 0.887
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 216.5 9
0081-0423
108.15 423.49 312 66.77 0.879
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 229.4 9
0082-0422
136.76 422.43 323 67.84 0.882
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 314.8 9
0083-0431
926.94 431.35 264 63.70 0.818
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 260.1 9
0084-0436
663.61 435.45 326 68.77 0.866
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 283.9 9
0085-0441
846.39 442.19 308 68.67 0.821
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 229.2 9
0086-0453
788.02 452.55 346 70.43 0.877
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 212.7 9
0087-0457
627.33 457.38 328 68.18 0.887
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 333.3 9
0088-0460
250.17 459.58 271 62.18 0.881