Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 276.5 9
0010-0056
 198.24 54.50 521 106.81 0.574
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 276.7 9
0011-0053
 750.74 53.84 362 71.60 0.887
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 274.1 9
0012-0058
 180.23 60.22 233 72.08 0.564
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 209.8 9
0013-0059
 124.69 58.76 368 74.67 0.829
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 259.4 9
0014-0065
 905.51 65.74 347 70.43 0.879
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 293.1 9
0015-0067
 424.89 67.01 396 75.01 0.884
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 296.5 9
0016-0068
 807.95 68.14 355 68.77 0.943
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 325.5 9
0017-0080
 716.35 80.08 379 75.36 0.839
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 306.2 9
0018-0076
 289.50 76.64 363 72.43 0.870
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 216.3 9
0019-0079
 840.09 78.86 418 77.26 0.880
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 586.2 9
0020-0082
 469.22 81.80 358 71.60 0.878
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 305.0 9
0021-0086
 533.45 85.87 390 75.84 0.852
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 393.2 9
0022-0091
 923.79 90.75 578 92.57 0.848
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 205.3 9
0023-0090
 79.93 89.86 378 73.50 0.879
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 274.8 9
0024-0097
 361.02 97.13 367 73.26 0.859
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 215.7 9
0025-0100
 832.20 99.85 407 77.84 0.844
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 226.3 9
0026-0104
 944.59 104.47 361 71.84 0.879
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 553.1 9
0027-0114
 400.72 115.10 887 115.98 0.829
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 279.1 9
0028-0113
 501.62 112.70 613 91.74 0.915
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 235.5 9
0029-0124
 118.68 124.31 366 71.60 0.897
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 246.0 9
0030-0133
 74.11 134.00 385 77.60 0.803
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 243.9 9
0031-0136
 215.57 136.49 367 71.60 0.900
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 248.6 9
0032-0137
 304.29 137.25 382 75.26 0.848
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 225.5 9
0033-0142
 849.97 142.28 397 76.67 0.849
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 312.4 9
0034-0153
 773.45 152.25 340 70.33 0.864