Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 349.0 3
0206-0359
402.75 359.21 322 65.94 0.931
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 312.4 3
0207-0355
684.95 355.37 203 55.70 0.822
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 290.0 3
0208-0357
483.80 358.09 169 51.11 0.813
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 608.3 3
0209-0358
112.39 358.32 148 45.46 0.900
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 315.4 3
0210-0367
589.51 368.23 600 167.82 0.268
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 456.3 3
0211-0365
513.79 364.74 343 70.43 0.869
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 364.9 3
0212-0362
928.13 361.92 149 49.70 0.758
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 285.1 3
0213-0365
165.18 365.39 230 59.11 0.827
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 291.5 3
0214-0369
26.07 370.68 286 71.01 0.713
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 354.4 3
0215-0367
315.45 367.37 307 66.43 0.874
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 479.7 3
0216-0367
651.27 367.38 319 68.18 0.862
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 356.8 3
0217-0371
910.25 370.76 309 70.43 0.783
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 435.8 3
0218-0371
875.06 370.77 305 66.43 0.869
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 304.5 3
0219-0371
374.83 371.79 255 60.77 0.868
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 369.2 3
0220-0374
263.29 374.75 348 71.26 0.861
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 427.5 3
0221-0377
712.73 377.14 273 63.36 0.855
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 295.1 3
0222-0379
762.43 379.09 281 65.01 0.835
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 342.0 3
0223-0379
841.85 379.72 226 58.77 0.822
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 291.6 3
0224-0379
147.84 379.00 276 64.77 0.827
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 261.0 3
0225-0383
54.65 383.69 298 68.18 0.805
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 403.9 3
0226-0382
546.20 382.45 304 65.36 0.894
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 371.0 3
0227-0383
352.41 382.21 226 57.60 0.856
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 403.5 3
0228-0382
503.03 382.35 258 60.18 0.895
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 274.3 3
0229-0382
89.21 382.66 219 53.70 0.954
COLO 858 Vemurafenib
0
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 369.0 3
0230-0384
103.89 384.05 200 57.11 0.771