Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 224.3 4
0177-0638
 333.96 638.20 438 79.74 0.866
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 260.3 4
0178-0639
 645.90 638.83 415 75.84 0.907
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 278.8 4
0179-0644
 254.30 643.97 410 75.84 0.896
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 271.3 4
0180-0644
 766.71 644.46 248 87.50 0.407
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 233.4 4
0181-0649
 475.70 648.79 796 111.64 0.803
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 282.4 4
0182-0654
 885.78 655.00 352 69.60 0.913
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 214.8 4
0183-0656
 36.38 656.07 390 73.26 0.913
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 187.4 4
0184-0658
 820.16 658.20 417 75.84 0.911
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 285.2 4
0185-0666
 109.47 665.99 445 80.91 0.854
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 209.3 4
0186-0668
 931.79 667.74 379 73.84 0.873
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 244.2 4
0187-0670
 764.69 670.90 426 79.74 0.842
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 209.2 4
0188-0670
 515.22 671.01 430 81.40 0.816
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 281.2 4
0189-0672
 228.59 672.38 446 80.67 0.861
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 217.9 4
0190-0677
 711.19 677.12 407 78.33 0.834
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 234.1 4
0191-0680
 362.66 680.49 407 74.18 0.929
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 224.9 4
0192-0682
 674.03 681.82 403 75.01 0.900
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 218.8 4
0193-0688
 31.90 688.55 387 75.60 0.851
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 177.2 4
0194-0700
 913.09 698.64 422 81.15 0.805
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 221.0 4
0195-0699
 177.40 699.16 425 77.01 0.900
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 187.7 4
0196-0706
 896.96 706.45 370 76.67 0.791
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 334.5 4
0197-0705
 451.24 705.04 393 73.01 0.926
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 256.8 4
0198-0706
 841.19 706.47 391 73.84 0.901
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 262.4 5
0001-0036
 912.92 36.08 697 97.74 0.917
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 549.7 5
0002-0037
 400.71 36.02 190 56.53 0.747
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 299.1 5
0003-0042
 383.54 44.47 533 95.15 0.740