Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 239.3 2
0084-0310
 171.84 310.52 381 72.43 0.913
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 229.4 2
0085-0312
 544.73 312.04 408 74.18 0.932
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 231.9 2
0086-0313
 622.31 313.79 659 123.98 0.539
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 254.3 2
0087-0318
 272.44 318.88 401 73.36 0.936
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 238.7 2
0088-0327
 478.15 327.67 419 81.50 0.793
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 229.3 2
0089-0329
 142.75 328.87 383 71.60 0.939
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 374.3 2
0090-0336
 451.27 335.94 416 79.50 0.827
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 241.1 2
0091-0339
 252.17 339.52 404 75.84 0.883
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 349.9 2
0092-0342
 303.53 342.63 382 73.01 0.900
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 229.1 2
0093-0344
 403.85 343.81 406 78.91 0.819
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 349.3 2
0094-0347
 823.72 347.14 383 72.43 0.918
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 210.9 2
0095-0348
 69.00 348.26 383 74.43 0.869
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 268.6 2
0096-0348
 226.00 348.72 398 75.84 0.870
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 227.9 2
0097-0352
 33.51 352.30 399 76.91 0.848
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 428.1 2
0098-0351
 882.76 350.71 322 69.70 0.833
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 289.9 2
0099-0362
 151.49 362.52 394 74.67 0.888
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 229.7 2
0100-0367
 622.50 367.96 417 75.84 0.911
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 357.4 2
0101-0371
 347.12 371.36 439 78.67 0.891
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 262.3 2
0102-0372
 879.45 371.13 475 87.50 0.780
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 273.0 2
0103-0380
 574.82 380.03 402 76.08 0.873
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 258.6 2
0104-0390
 709.91 389.84 825 113.88 0.799
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 293.8 2
0105-0388
 95.79 388.54 386 75.50 0.851
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 324.3 2
0106-0388
 199.33 387.52 442 82.33 0.820
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 252.6 2
0107-0390
 526.13 389.65 420 80.57 0.813
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 268.3 2
0108-0401
 820.21 401.50 419 77.84 0.869