Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 218.0 9
0167-0499
 186.12 500.16 391 75.36 0.865
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 243.7 9
0168-0502
 514.94 502.30 403 74.77 0.906
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 254.1 9
0169-0509
 862.02 509.84 373 71.84 0.908
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 303.4 9
0170-0512
 468.13 511.72 397 75.26 0.881
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 277.7 9
0171-0513
 775.33 513.07 380 74.67 0.856
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 246.5 9
0172-0517
 556.44 516.30 415 79.50 0.825
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 265.5 9
0173-0520
 270.30 519.66 642 94.33 0.907
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 214.0 9
0174-0520
 181.41 520.11 444 82.18 0.826
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 231.3 9
0175-0532
 226.41 531.71 396 73.84 0.913
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 340.0 9
0176-0537
 542.74 538.48 362 74.77 0.814
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 281.1 9
0177-0540
 403.69 540.32 346 69.94 0.889
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 413.3 9
0178-0545
 561.00 545.00 297 67.60 0.817
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 230.3 9
0179-0544
 944.64 544.28 384 73.26 0.899
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 373.7 9
0180-0546
 841.98 546.34 389 72.43 0.932
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 298.7 9
0181-0550
 301.68 550.16 392 73.84 0.903
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 250.5 9
0182-0561
 415.75 561.58 694 106.23 0.773
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 551.9 9
0183-0555
 544.47 555.11 252 61.46 0.838
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 235.5 9
0184-0562
 514.95 562.28 417 76.67 0.891
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 258.6 9
0185-0569
 793.03 569.10 400 75.84 0.874
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 254.7 9
0186-0572
 258.47 571.14 339 70.91 0.847
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 271.7 9
0187-0582
 210.17 582.11 368 73.01 0.867
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 316.6 9
0188-0584
 273.44 585.56 492 85.88 0.838
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 231.2 9
0189-0583
 903.29 582.47 449 79.50 0.893
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 304.7 9
0190-0586
 660.06 585.62 380 73.84 0.876
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-1
 H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
 H3 (pS10) 298.8 9
0191-0595
 175.90 595.08 377 73.84 0.869