Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 209.4 6
0045-0148
301.61 148.60 373 73.50 0.868
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 246.1 6
0046-0148
505.48 148.16 399 77.01 0.845
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 265.5 6
0047-0149
838.37 149.10 369 72.67 0.878
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 246.9 6
0048-0153
190.42 152.90 356 80.43 0.692
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 246.3 6
0049-0159
767.90 159.50 381 75.01 0.851
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 237.5 6
0050-0159
605.24 159.53 411 76.67 0.879
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 219.0 6
0051-0163
326.95 162.93 371 72.43 0.889
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 265.2 6
0052-0162
560.70 162.97 397 76.67 0.849
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 217.9 6
0053-0165
928.56 164.82 391 73.01 0.922
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 233.4 6
0054-0167
685.73 167.59 399 73.60 0.926
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 245.8 6
0055-0167
474.63 166.86 391 76.18 0.847
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 220.3 6
0056-0171
364.60 171.26 369 73.26 0.864
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 318.6 6
0057-0177
70.64 177.11 396 78.18 0.814
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 260.5 6
0058-0178
578.94 178.43 399 75.84 0.872
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 474.0 6
0059-0178
93.26 177.67 356 71.94 0.864
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 245.9 6
0060-0180
519.28 180.24 369 71.26 0.913
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 475.5 6
0061-0188
811.84 187.98 356 71.60 0.873
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 257.8 6
0062-0188
732.47 188.22 376 73.26 0.880
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 285.3 6
0063-0188
398.67 187.91 374 72.77 0.888
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 263.4 6
0064-0188
459.42 188.42 391 75.36 0.865
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 271.2 6
0065-0192
707.38 192.26 387 74.43 0.878
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 267.9 6
0066-0194
832.11 193.10 379 75.84 0.828
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 244.3 6
0067-0201
129.67 201.14 384 77.26 0.809
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 222.4 6
0068-0203
551.30 203.08 376 73.50 0.875
COLO 858 Vemurafenib
0.1
uM
48
hr
image if available image if available
Cycle-1
H3 (pS10) rabbit IgG(H+L) / goat / cross-adsorbed
Cy3
H3 (pS10) 310.5 6
0069-0204
902.38 204.65 333 66.77 0.939