Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 266.4 3
0161-0395
 231.76 395.64 345 74.91 0.773
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 280.5 3
0162-0395
 819.43 395.72 362 71.01 0.902
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1718.2 3
0163-0396
 119.41 396.33 326 69.26 0.854
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 383.3 3
0164-0398
 477.43 398.21 310 64.77 0.929
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 490.8 3
0165-0399
 751.44 398.79 398 77.74 0.828
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 415.1 3
0166-0402
 304.30 401.92 380 73.60 0.882
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 233.6 3
0167-0410
 371.75 410.15 720 105.88 0.807
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 411.6 3
0168-0403
 438.20 403.57 370 74.08 0.847
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 260.7 3
0169-0421
 593.76 420.87 363 78.91 0.733
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 225.1 3
0170-0421
 718.80 421.57 380 77.15 0.802
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 278.5 3
0171-0423
 774.90 423.59 361 75.84 0.789
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 131.9 3
0172-0428
 33.62 428.69 325 72.91 0.768
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 189.0 3
0173-0431
 269.61 431.74 353 76.91 0.750
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 872.3 3
0174-0431
 528.13 432.17 372 77.94 0.770
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 340.9 3
0175-0436
 693.67 436.44 627 99.64 0.794
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 298.3 3
0176-0447
 421.89 446.54 748 106.95 0.822
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 299.8 3
0177-0440
 641.30 440.64 368 74.57 0.832
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1192.7 3
0178-0446
 162.63 445.35 712 105.88 0.798
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 830.4 3
0179-0443
 218.47 442.01 246 66.18 0.706
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 683.0 3
0180-0444
 231.04 446.37 219 63.70 0.678
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 159.6 3
0181-0448
 196.51 448.67 338 72.43 0.810
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 355.4 3
0182-0447
 315.10 447.79 367 74.91 0.822
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 229.6 3
0183-0452
 248.41 451.51 349 71.60 0.856
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 475.0 3
0184-0455
 527.89 453.68 409 81.84 0.767
COLO 858 Vemurafenib
0.1
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 703.6 3
0185-0461
 105.25 461.70 375 81.15 0.716