Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 272.7 6
0156-0334
 415.15 333.75 322 69.26 0.844
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1230.5 6
0157-0336
 57.05 335.97 361 70.18 0.921
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 362.8 6
0158-0336
 705.21 336.45 327 70.18 0.834
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 3087.9 6
0159-0337
 247.85 337.10 231 57.94 0.865
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 931.0 6
0160-0338
 873.94 339.03 219 55.80 0.884
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1123.8 6
0161-0346
 319.24 345.76 246 62.28 0.797
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1368.0 6
0162-0347
 649.01 347.45 361 70.43 0.915
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 3404.9 6
0163-0346
 262.33 345.89 274 62.77 0.874
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1576.6 6
0164-0346
 195.54 347.46 207 57.94 0.775
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 3577.1 6
0165-0349
 751.50 349.29 219 55.94 0.879
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 2435.8 6
0166-0351
 433.65 351.56 202 55.94 0.811
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 3571.2 6
0167-0355
 275.20 356.19 224 54.77 0.938
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 2202.7 6
0168-0358
 330.88 357.70 234 60.18 0.812
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1720.8 6
0169-0358
 510.68 358.42 247 66.67 0.698
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1049.5 6
0170-0353
 874.29 352.74 227 58.28 0.840
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 471.0 6
0171-0360
 458.55 360.08 306 69.94 0.786
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 305.2 6
0172-0356
 782.57 356.41 305 64.77 0.914
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1837.9 6
0173-0359
 612.70 359.17 310 65.01 0.922
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 336.4 6
0174-0360
 19.86 360.13 273 65.84 0.791
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 3607.5 6
0175-0362
 759.16 362.11 203 55.94 0.815
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 296.8 6
0176-0365
 107.78 365.12 297 67.36 0.823
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1323.4 6
0177-0369
 650.63 368.91 309 65.36 0.909
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 3322.1 6
0178-0368
 427.61 368.25 236 56.87 0.917
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 516.6 6
0179-0370
 895.90 370.77 278 63.60 0.864
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 647.3 6
0180-0371
 917.35 371.10 266 62.43 0.858