Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 373.1 4
0072-0173
 930.06 173.44 334 73.60 0.775
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 2251.8 4
0073-0171
 572.45 171.06 277 65.01 0.824
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 413.1 4
0074-0175
 117.62 175.02 347 70.18 0.885
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 5887.5 4
0075-0174
 608.69 174.77 343 72.67 0.816
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 6898.0 4
0076-0176
 709.60 175.55 246 58.77 0.895
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 753.0 4
0077-0182
 743.35 181.60 249 57.94 0.932
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 390.4 4
0078-0187
 260.34 187.52 305 68.43 0.819
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 4306.2 4
0079-0187
 396.30 186.68 288 65.01 0.856
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1256.6 4
0080-0189
 524.11 189.77 271 62.53 0.871
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 783.2 4
0081-0192
 303.68 191.71 332 70.77 0.833
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1035.5 4
0082-0193
 77.16 194.14 250 58.87 0.906
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1913.1 4
0083-0204
 189.14 203.70 711 106.91 0.782
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1286.4 4
0084-0195
 564.72 195.48 236 56.53 0.928
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 7990.8 4
0085-0197
 603.83 197.14 195 51.70 0.917
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 586.0 4
0086-0201
 361.27 201.37 278 62.18 0.903
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 2409.4 4
0087-0203
 910.95 203.03 256 64.53 0.773
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 5957.9 4
0088-0200
 621.21 201.45 183 52.53 0.833
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 2364.1 4
0089-0208
 451.61 208.79 227 59.94 0.794
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 3610.7 4
0090-0205
 142.26 204.59 219 54.53 0.926
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1028.5 4
0091-0211
 77.12 210.81 245 58.63 0.896
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1450.7 4
0092-0214
 212.55 213.45 279 62.53 0.897
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 531.3 4
0093-0215
 882.23 215.37 291 63.36 0.911
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1505.2 4
0094-0218
 788.07 218.22 272 63.94 0.836
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 278.7 4
0095-0220
 822.27 219.43 310 65.36 0.912
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 861.2 4
0096-0221
 236.96 221.31 270 60.18 0.937