Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 792.6 2
0258-0512
 475.01 511.55 298 65.60 0.870
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1487.4 2
0259-0513
 867.81 513.90 265 61.36 0.885
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1131.3 2
0260-0516
 850.85 516.77 257 59.60 0.909
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 592.5 2
0261-0516
 146.95 516.06 263 59.36 0.938
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1461.6 2
0262-0523
 580.94 523.79 157 47.94 0.858
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 378.6 2
0263-0524
 902.61 524.32 334 71.01 0.832
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 511.0 2
0264-0524
 171.12 524.21 247 58.53 0.906
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 3611.3 2
0265-0529
 743.25 528.55 460 81.26 0.876
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 701.2 2
0266-0529
 338.24 529.73 255 61.36 0.851
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 435.7 2
0267-0531
 547.18 530.79 348 70.43 0.882
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1427.5 2
0268-0532
 520.09 532.34 249 59.60 0.881
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 930.2 2
0269-0534
 822.04 534.95 290 65.36 0.853
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 347.4 2
0270-0536
 121.32 535.69 443 79.84 0.873
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 387.7 2
0271-0536
 253.91 537.11 303 64.77 0.908
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 438.3 2
0272-0540
 73.04 539.97 266 60.43 0.915
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 252.8 2
0273-0540
 438.67 540.09 274 60.53 0.940
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 620.9 2
0274-0543
 474.92 543.22 497 82.43 0.919
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 950.8 2
0275-0543
 495.38 542.73 251 57.94 0.940
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 475.2 2
0276-0545
 179.70 545.24 306 69.94 0.786
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 786.7 2
0277-0548
 795.48 548.99 303 64.43 0.917
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1723.4 2
0278-0551
 945.99 550.84 288 62.53 0.926
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 386.6 2
0279-0553
 24.96 553.10 285 62.18 0.926
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 2108.8 2
0280-0557
 618.00 556.84 266 63.11 0.839
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 2698.2 2
0281-0561
 641.94 560.82 711 101.98 0.859
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-2
 S6 (pS240; pS244) / Alexa Fluor 488 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 S6 (pS240; pS244) 1134.0 2
0282-0560
 291.60 560.59 518 83.01 0.945