Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 405.6 5
0043-0527
 286.51 526.75 220 61.01 0.743
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 608.6 5
0044-0535
 622.69 534.63 187 56.28 0.742
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 500.3 5
0045-0550
 220.89 550.54 202 57.94 0.756
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 580.0 5
0046-0548
 249.45 548.43 172 52.28 0.791
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 606.7 5
0047-0563
 153.70 562.95 110 53.36 0.486
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 370.8 5
0048-0583
 634.62 583.75 264 63.84 0.814
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 893.8 5
0049-0594
 129.69 594.89 191 54.18 0.818
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 803.9 5
0050-0606
 526.68 606.72 254 62.53 0.816
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 487.3 5
0051-0621
 548.39 620.38 261 60.53 0.895
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1153.7 5
0052-0633
 394.79 633.43 175 57.94 0.655
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 813.9 5
0053-0649
 879.38 648.46 267 62.77 0.852
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 404.9 5
0054-0658
 86.40 658.34 208 66.04 0.599
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 478.3 5
0055-0659
 146.43 658.61 215 57.11 0.828
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1079.3 5
0056-0676
 660.44 675.54 242 63.36 0.758
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 358.0 5
0057-0680
 392.72 679.46 237 61.94 0.776
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1012.7 5
0058-0686
 413.76 685.86 186 51.70 0.875
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1191.0 5
0059-0701
 377.39 701.40 197 55.70 0.798
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 783.7 5
0060-0703
 482.30 702.70 182 64.67 0.547
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 557.2 6
0001-0056
 930.28 56.78 245 59.11 0.881
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1577.4 6
0002-0062
 146.01 62.35 215 56.77 0.838
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 380.3 6
0003-0073
 880.87 72.50 278 64.43 0.842
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 501.9 6
0004-0082
 78.92 81.60 182 51.94 0.848
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 438.3 6
0005-0093
 752.88 94.14 288 67.01 0.806
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1371.5 6
0006-0112
 641.80 112.21 197 57.60 0.746
COLO 858 Vemurafenib
3.16
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 675.4 6
0007-0120
 317.19 120.41 213 53.11 0.949