Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 605.1 9
0078-0401
 429.53 401.57 283 62.43 0.913
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 537.1 9
0079-0413
 477.40 413.11 239 58.53 0.877
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 371.7 9
0080-0418
 752.22 417.97 326 67.94 0.887
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1149.1 9
0081-0423
 108.15 423.49 312 66.77 0.879
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 296.8 9
0082-0422
 136.76 422.43 323 67.84 0.882
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 428.3 9
0083-0431
 926.94 431.35 264 63.70 0.818
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 393.5 9
0084-0436
 663.61 435.45 326 68.77 0.866
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 389.3 9
0085-0441
 846.39 442.19 308 68.67 0.821
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 712.9 9
0086-0453
 788.02 452.55 346 70.43 0.877
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 329.6 9
0087-0457
 627.33 457.38 328 68.18 0.887
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 669.0 9
0088-0460
 250.17 459.58 271 62.18 0.881
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 749.0 9
0089-0460
 360.06 459.62 272 61.36 0.908
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 850.4 9
0090-0466
 762.09 465.46 350 71.01 0.872
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 461.6 9
0091-0473
 583.58 473.73 297 64.18 0.906
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 589.2 9
0092-0483
 354.63 482.60 295 64.18 0.900
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 440.4 9
0093-0490
 838.00 490.06 300 64.77 0.899
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 295.0 9
0094-0496
 656.41 496.27 342 72.53 0.817
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 484.6 9
0095-0495
 418.64 495.14 284 62.77 0.906
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 317.0 9
0096-0498
 792.53 497.76 331 69.36 0.865
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 654.4 9
0097-0496
 774.17 495.84 350 71.94 0.850
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 374.7 9
0098-0499
 52.74 498.30 283 62.43 0.913
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1723.0 9
0099-0501
 727.06 500.76 271 61.94 0.888
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 759.5 9
0100-0505
 207.71 504.91 249 58.18 0.924
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1619.8 9
0101-0521
 78.62 521.30 261 65.01 0.776
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 298.5 9
0102-0521
 614.23 521.50 371 70.18 0.946