Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 616.7 7
0055-0352
 133.12 352.78 247 64.43 0.748
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 320.9 7
0056-0355
 729.67 355.23 293 63.36 0.917
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 603.3 7
0057-0366
 284.61 366.61 267 59.70 0.941
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 530.0 7
0058-0377
 900.04 377.04 305 72.18 0.736
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 377.8 7
0059-0377
 130.33 376.64 342 70.08 0.875
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 636.9 7
0060-0382
 614.20 382.79 380 73.60 0.882
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 375.8 7
0061-0386
 419.80 385.79 329 69.84 0.848
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 375.9 7
0062-0384
 562.34 383.61 317 66.18 0.909
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 291.5 7
0063-0392
 845.59 392.78 308 67.01 0.862
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 910.7 7
0064-0396
 676.19 396.86 321 68.18 0.868
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 321.5 7
0065-0397
 781.94 396.61 342 69.84 0.881
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 397.9 7
0066-0399
 58.99 398.31 273 63.60 0.848
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 295.9 7
0067-0409
 352.76 410.17 340 72.67 0.809
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 462.9 7
0068-0417
 292.67 417.10 285 67.84 0.778
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 320.6 7
0069-0423
 37.19 423.02 275 69.26 0.721
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 517.6 7
0070-0419
 533.04 419.50 266 63.70 0.824
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 493.0 7
0071-0426
 229.90 425.89 293 66.43 0.834
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 487.7 7
0072-0438
 885.79 438.02 319 70.77 0.800
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 638.6 7
0073-0437
 138.79 436.51 217 59.94 0.759
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 328.8 7
0074-0443
 717.99 443.46 337 69.60 0.874
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 370.8 7
0075-0448
 449.95 447.65 306 67.84 0.836
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 889.5 7
0076-0447
 484.62 447.15 225 58.18 0.835
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 555.0 7
0077-0453
 631.77 455.68 387 93.98 0.551
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 473.7 7
0078-0449
 298.81 448.89 269 66.43 0.766
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 753.6 7
0079-0468
 104.09 467.16 106 44.53 0.672