Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 461.2 4
0106-0617
 891.27 617.43 316 67.36 0.875
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1007.8 4
0107-0628
 95.11 628.12 255 61.60 0.845
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 742.4 4
0108-0631
 374.02 631.06 239 59.11 0.860
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 560.7 4
0109-0636
 235.67 635.97 293 68.77 0.779
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 445.3 4
0110-0634
 316.13 633.75 228 58.87 0.827
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 250.7 4
0111-0647
 665.33 646.45 365 72.18 0.880
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 345.0 4
0112-0653
 495.85 653.47 310 65.36 0.912
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 455.3 4
0113-0659
 788.18 658.88 344 69.84 0.886
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 262.2 4
0114-0659
 552.10 659.61 283 67.50 0.781
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 452.0 4
0115-0666
 282.01 665.44 321 67.60 0.883
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 433.6 4
0116-0668
 727.49 667.81 322 67.60 0.886
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 453.6 4
0117-0680
 397.53 679.43 239 57.94 0.895
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 432.9 4
0118-0686
 292.84 686.66 270 61.36 0.901
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 356.4 4
0119-0695
 861.38 694.59 324 73.50 0.754
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 865.7 4
0120-0698
 161.50 698.70 379 76.53 0.813
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 476.4 4
0121-0700
 903.67 700.99 382 111.88 0.383
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1002.6 4
0122-0702
 144.98 702.79 376 75.94 0.819
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 743.9 4
0123-0699
 828.87 699.10 217 57.60 0.822
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 585.6 4
0124-0700
 594.34 699.65 244 60.43 0.840
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 301.2 4
0125-0701
 642.94 700.49 282 62.77 0.899
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 331.1 5
0001-0034
 395.08 34.47 406 75.84 0.887
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 361.1 5
0002-0038
 646.42 38.21 370 70.77 0.928
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 379.9 5
0003-0036
 895.51 36.62 132 44.87 0.824
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 456.1 5
0004-0053
 63.91 53.11 294 64.18 0.897
COLO 858 Vemurafenib
1
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 396.1 5
0005-0056
 130.66 56.10 288 64.77 0.863