Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 633.5 3
0037-0237
281.30 237.36 268 63.11 0.845
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 413.9 3
0038-0255
675.34 254.68 236 58.53 0.866
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 737.4 3
0039-0258
799.14 258.45 311 66.43 0.886
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 4445.2 3
0040-0262
553.47 262.69 241 58.53 0.884
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 781.9 3
0041-0266
25.16 265.73 251 63.01 0.794
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 831.3 3
0042-0265
863.12 265.41 274 63.60 0.851
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 516.8 3
0043-0265
141.87 264.90 244 59.94 0.853
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 2927.4 3
0044-0269
487.64 268.24 132 54.77 0.553
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3471.0 3
0045-0276
280.84 276.59 386 73.26 0.904
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 969.1 3
0046-0283
412.01 282.93 270 61.36 0.901
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1072.1 3
0047-0287
480.67 286.13 285 76.33 0.615
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 459.5 3
0048-0285
614.69 285.54 353 71.01 0.880
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 307.0 3
0049-0296
198.32 296.29 316 68.77 0.840
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 545.3 3
0050-0294
808.99 294.66 306 65.84 0.887
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 4600.1 3
0051-0300
332.45 299.79 423 75.84 0.924
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 337.3 3
0052-0300
512.00 299.74 300 64.77 0.899
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 4138.1 3
0053-0297
560.29 297.60 266 59.70 0.938
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 509.5 3
0054-0305
631.55 304.42 320 68.18 0.865
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 416.1 3
0055-0306
733.69 306.77 268 63.26 0.842
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 628.6 3
0056-0305
416.53 305.25 160 48.63 0.850
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 3457.1 3
0057-0317
101.43 316.75 262 61.60 0.868
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 256.6 3
0058-0318
58.86 318.20 366 72.08 0.885
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 359.3 3
0059-0321
912.88 321.82 329 68.77 0.874
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 775.6 3
0060-0321
412.84 321.14 247 64.77 0.740
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 338.5 3
0061-0347
476.90 347.05 328 68.67 0.874