Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 664.4 2
0067-0342
868.58 341.35 293 64.18 0.894
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 359.2 2
0068-0348
366.56 348.52 336 68.18 0.908
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 437.7 2
0069-0351
922.88 351.54 356 73.01 0.839
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 427.9 2
0070-0356
941.20 356.11 350 71.60 0.858
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 661.7 2
0071-0357
153.69 357.39 612 108.23 0.657
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1322.2 2
0072-0374
406.60 374.81 483 84.43 0.852
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1233.3 2
0073-0383
289.40 383.00 313 67.94 0.852
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 638.9 2
0074-0383
102.55 383.68 245 59.36 0.874
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 561.1 2
0075-0385
593.84 385.36 304 67.84 0.830
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 585.2 2
0076-0387
356.28 387.46 294 66.43 0.837
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 661.5 2
0077-0393
141.79 393.65 318 69.26 0.833
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 866.2 2
0078-0397
212.63 398.04 322 65.60 0.940
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 326.6 2
0079-0404
652.55 404.88 359 70.77 0.901
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 385.1 2
0080-0409
431.96 408.57 379 72.67 0.902
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 642.9 2
0081-0430
485.77 430.66 314 68.18 0.849
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 896.7 2
0082-0436
193.76 436.75 316 68.43 0.848
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 634.1 2
0083-0436
587.81 435.82 323 69.01 0.852
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 422.0 2
0084-0437
42.06 436.96 271 61.11 0.912
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1660.3 2
0085-0437
821.66 437.77 223 56.77 0.870
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1448.4 2
0086-0448
660.63 448.83 239 58.53 0.877
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 311.0 2
0087-0453
54.39 453.00 355 70.77 0.891
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 442.7 2
0088-0459
340.67 458.85 249 61.84 0.818
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1225.2 2
0089-0458
740.67 458.64 236 59.70 0.832
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 383.8 2
0090-0467
519.60 467.21 345 69.36 0.901
COLO 858 Vemurafenib
1
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 566.4 2
0091-0479
573.14 478.52 194 66.43 0.552