Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 8298.4 8
0168-0549
 741.84 549.81 294 64.18 0.897
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 4037.1 8
0169-0552
 689.00 552.49 408 78.18 0.839
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 682.6 8
0170-0554
 612.03 554.10 392 73.84 0.903
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 787.0 8
0171-0558
 572.27 558.01 387 73.01 0.912
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 265.0 8
0172-0558
 818.49 558.24 379 72.67 0.902
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 270.4 8
0173-0559
 239.19 559.34 388 74.08 0.888
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 341.4 8
0174-0567
 401.42 566.65 432 77.01 0.915
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 267.1 8
0175-0569
 72.52 568.92 371 71.84 0.903
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 255.0 8
0176-0568
 115.15 567.72 380 74.08 0.870
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 286.5 8
0177-0571
 928.14 571.53 366 71.01 0.912
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 305.6 8
0178-0573
 894.54 573.35 385 73.26 0.902
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 421.1 8
0179-0573
 472.87 572.73 373 71.60 0.914
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 413.4 8
0180-0578
 365.59 578.38 422 77.94 0.873
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 660.4 8
0181-0577
 620.21 577.65 398 74.43 0.903
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 254.4 8
0182-0579
 657.58 579.15 397 75.50 0.875
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 284.9 8
0183-0579
 130.71 579.51 295 63.84 0.910
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 367.3 8
0184-0586
 870.14 586.32 352 70.43 0.892
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 350.5 8
0185-0593
 306.08 592.85 380 72.43 0.910
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 279.1 8
0186-0591
 574.66 591.46 375 72.43 0.898
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 244.5 8
0187-0600
 798.09 600.44 796 109.88 0.828
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 371.3 8
0188-0598
 344.16 598.08 407 76.08 0.884
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 380.5 8
0189-0598
 649.17 599.12 377 74.67 0.850
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 777.9 8
0190-0606
 189.49 605.77 406 77.11 0.858
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 727.7 8
0191-0611
 212.45 611.06 398 76.77 0.849
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 415.0 8
0192-0609
 498.57 609.64 381 75.01 0.851