Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 612.7 7
0171-0605
 117.62 605.11 407 76.67 0.870
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 381.4 7
0172-0608
 251.39 608.26 407 75.26 0.903
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 539.6 7
0173-0609
 171.24 609.16 393 73.01 0.926
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 284.9 7
0174-0609
 623.41 609.05 462 82.08 0.862
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 282.3 7
0175-0622
 508.45 622.39 442 79.26 0.884
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 753.8 7
0176-0622
 537.13 621.59 421 76.08 0.914
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 242.1 7
0177-0629
 839.20 629.32 388 73.60 0.900
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 435.1 7
0178-0630
 458.46 629.99 392 73.84 0.903
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 612.8 7
0179-0630
 375.06 630.96 424 77.84 0.879
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 366.1 7
0180-0634
 120.29 634.40 434 83.15 0.789
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 381.2 7
0181-0638
 566.69 638.48 419 78.43 0.856
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 599.1 7
0182-0643
 82.73 643.20 390 75.50 0.860
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 305.8 7
0183-0641
 31.35 641.60 353 68.77 0.938
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 393.9 7
0184-0645
 289.37 645.10 406 73.60 0.942
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 304.5 7
0185-0644
 599.43 644.18 456 81.50 0.863
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 454.1 7
0186-0648
 147.44 647.92 408 74.18 0.932
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 327.3 7
0187-0651
 710.26 650.69 404 75.84 0.883
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 367.2 7
0188-0653
 250.53 653.75 401 76.08 0.871
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 482.4 7
0189-0657
 392.64 657.16 439 78.43 0.897
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 445.7 7
0190-0667
 804.84 668.45 855 117.30 0.781
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 364.7 7
0191-0664
 204.68 664.59 410 75.01 0.916
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 410.4 7
0192-0667
 461.60 667.58 383 72.43 0.918
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1039.2 7
0193-0668
 870.91 668.58 360 70.43 0.912
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 508.1 7
0194-0671
 620.80 671.53 382 72.43 0.915
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 453.7 7
0195-0682
 727.61 681.81 759 105.74 0.853