Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1335.7 6
0171-0696
 775.38 696.07 302 63.36 0.945
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 382.2 6
0172-0701
 605.07 701.16 380 72.18 0.916
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 377.3 6
0173-0702
 95.54 701.63 451 85.60 0.773
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 541.4 6
0174-0704
 120.66 703.75 188 52.63 0.853
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 279.4 6
0175-0709
 319.30 709.24 414 81.01 0.793
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 416.0 7
0001-0031
 448.84 30.88 428 78.08 0.882
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1965.5 7
0002-0032
 838.72 31.96 384 75.50 0.847
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 505.6 7
0003-0036
 597.08 35.70 508 86.43 0.855
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1093.9 7
0004-0040
 660.75 40.02 389 73.26 0.911
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 351.6 7
0005-0043
 46.37 43.18 436 80.08 0.854
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 461.4 7
0006-0046
 171.75 45.61 395 76.18 0.855
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1304.3 7
0007-0056
 779.49 56.58 762 105.40 0.862
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 690.2 7
0008-0053
 366.46 52.98 413 77.84 0.857
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 931.9 7
0009-0061
 736.50 60.83 821 110.33 0.848
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 333.0 7
0010-0059
 514.63 59.64 469 80.67 0.906
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 841.0 7
0011-0060
 622.88 60.55 447 81.01 0.856
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 628.8 7
0012-0062
 112.13 62.35 400 73.84 0.922
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 371.8 7
0013-0064
 827.38 64.14 397 73.01 0.936
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 544.2 7
0014-0065
 209.40 65.33 397 75.26 0.881
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 396.5 7
0015-0068
 474.31 68.46 445 77.84 0.923
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 492.8 7
0016-0073
 759.50 73.09 441 82.33 0.818
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 505.1 7
0017-0071
 25.96 71.16 395 74.43 0.896
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 486.2 7
0018-0075
 168.45 75.59 400 75.26 0.888
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 337.3 7
0019-0076
 649.96 75.19 460 84.91 0.802
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 842.5 7
0020-0076
 914.47 76.08 389 74.67 0.877