Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 342.2 6
0121-0488
817.96 488.82 421 77.84 0.873
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 337.1 6
0122-0487
700.13 487.33 456 79.60 0.904
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 417.0 6
0123-0494
467.40 493.91 413 75.01 0.922
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 391.5 6
0124-0497
519.65 497.45 378 73.01 0.891
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 1082.4 6
0125-0503
71.84 502.98 397 73.60 0.921
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 331.4 6
0126-0510
312.54 510.67 394 76.91 0.837
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 310.6 6
0127-0525
839.74 525.65 437 78.43 0.893
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 308.3 6
0128-0527
540.65 527.67 412 75.26 0.914
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 390.9 6
0129-0528
685.34 528.07 370 72.43 0.886
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 356.5 6
0130-0531
408.66 531.66 427 82.91 0.781
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 275.0 6
0131-0545
184.59 545.56 423 77.26 0.891
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 334.1 6
0132-0550
783.64 550.56 434 78.67 0.881
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 313.7 6
0133-0554
16.21 553.55 343 69.84 0.884
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 244.0 6
0134-0558
889.37 558.36 399 75.26 0.885
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 344.6 6
0135-0563
292.95 563.04 416 75.01 0.929
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 326.0 6
0136-0566
246.05 566.66 403 75.26 0.894
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 293.4 6
0137-0574
207.12 574.39 383 73.01 0.903
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 498.5 6
0138-0580
407.06 580.07 744 102.91 0.883
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 808.4 6
0139-0579
123.64 579.43 381 73.84 0.878
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 719.0 6
0140-0584
648.20 584.41 298 79.26 0.596
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 303.1 6
0141-0588
340.02 588.45 389 76.08 0.844
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 294.8 6
0142-0591
512.96 591.21 414 76.08 0.899
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 315.7 6
0143-0592
930.00 592.72 460 79.84 0.907
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 279.2 6
0144-0600
604.74 599.99 396 74.67 0.893
COLO 858 Vemurafenib
0.316
uM
48
hr
image if available image if available
Cycle-1
KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
KI-67 422.3 6
0145-0610
674.51 609.31 411 75.26 0.912