Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 688.2 4
0066-0235
 390.33 235.47 392 75.50 0.864
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 308.2 4
0067-0243
 108.57 243.69 401 77.50 0.839
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 315.4 4
0068-0246
 838.49 246.20 452 80.91 0.868
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 301.4 4
0069-0248
 543.64 247.86 431 78.43 0.881
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 624.1 4
0070-0248
 753.37 248.95 404 77.50 0.845
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1277.4 4
0071-0248
 451.53 248.74 468 84.77 0.818
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 322.6 4
0072-0252
 323.73 252.20 406 77.26 0.855
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 320.2 4
0073-0254
 473.20 254.32 420 80.18 0.821
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 396.9 4
0074-0255
 912.05 255.95 362 71.26 0.896
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 364.3 4
0075-0258
 640.31 258.51 486 85.50 0.835
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 326.4 4
0076-0260
 665.44 259.77 452 83.50 0.815
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 566.0 4
0077-0263
 872.41 263.11 474 83.50 0.854
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 366.9 4
0078-0266
 367.06 265.64 415 76.43 0.893
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 444.1 4
0079-0268
 780.52 268.02 378 73.26 0.885
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 315.0 4
0080-0274
 179.37 274.15 430 77.01 0.911
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 597.5 4
0081-0280
 251.34 280.48 399 75.26 0.885
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 408.5 4
0082-0286
 526.38 286.29 418 77.84 0.867
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2766.6 4
0083-0291
 805.27 290.90 691 98.57 0.894
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 369.7 4
0084-0289
 415.43 289.20 429 80.08 0.841
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 318.2 4
0085-0303
 165.61 303.77 430 77.84 0.892
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 348.1 4
0086-0308
 709.10 308.30 472 83.26 0.856
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 304.5 4
0087-0311
 288.03 311.72 406 79.50 0.807
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 279.6 4
0088-0312
 901.72 311.94 422 76.43 0.908
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 628.0 4
0089-0318
 685.82 317.83 441 80.67 0.852
COLO 858 Vemurafenib
0.316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2750.5 4
0090-0323
 249.96 323.02 742 104.47 0.854